About the Authors

Michelle L. Scalley-Kim

Affiliation Department of Protein Engineering, VLST Corporation, Seattle, Washington, United States of America

Bruce W. Hess

Affiliation Department of Immunology and Preclinical Pharmacology, VLST Corporation, Seattle, Washington, United States of America

Ryan L. Kelly

Affiliation Department of Protein Sciences, VLST Corporation, Seattle, Washington, United States of America

Anne-Rachel F. Krostag

Current address: Department of Molecular Networks, Allen Institute for Brain Science, Seattle, Washington, United States of America

Affiliation Department of Protein Engineering, VLST Corporation, Seattle, Washington, United States of America

Kurt H. Lustig

Affiliation Department of Immunology and Preclinical Pharmacology, VLST Corporation, Seattle, Washington, United States of America

John S. Marken

Affiliation Department of Protein Engineering, VLST Corporation, Seattle, Washington, United States of America

Pamela J. Ovendale

Affiliation Department of Immunology and Preclinical Pharmacology, VLST Corporation, Seattle, Washington, United States of America

Aaron R. Posey

Affiliation Department of Immunology and Preclinical Pharmacology, VLST Corporation, Seattle, Washington, United States of America

Pamela J. Smolak

Affiliation Department of Protein Engineering, VLST Corporation, Seattle, Washington, United States of America

Janelle D. L. Taylor

Current address: Department of Research, Oncothyreon Inc., Seattle, Washington, United States of America

Affiliation Department of Protein Engineering, VLST Corporation, Seattle, Washington, United States of America

C. L. Wood

Current address: Department of Discovery Research, Omeros Corporation, Seattle, Washington, United States of America

Affiliation Department of Protein Engineering, VLST Corporation, Seattle, Washington, United States of America

David L. Bienvenue

Affiliation Department of Protein Sciences, VLST Corporation, Seattle, Washington, United States of America

Peter Probst

Affiliation Department of Immunology and Preclinical Pharmacology, VLST Corporation, Seattle, Washington, United States of America

Ruth A. Salmon

Affiliation Department of Immunology and Preclinical Pharmacology, VLST Corporation, Seattle, Washington, United States of America

Daniel S. Allison

Current address: Department of Antibody Technologies, Alder Biopharmaceuticals Inc., Bothell, Washington, United States of America

Affiliation Department of Protein Engineering, VLST Corporation, Seattle, Washington, United States of America

Teresa M. Foy

tfoy@vlstcorp.com

Affiliation Department of Immunology and Preclinical Pharmacology, VLST Corporation, Seattle, Washington, United States of America

Carol J. Raport

Affiliation Department of Immunology and Preclinical Pharmacology, VLST Corporation, Seattle, Washington, United States of America

Competing Interests

The authors have read the journal’s policy and have the following conflicts: Dr. Foy is an employee of VLST Corporation and they provided funding for this study. This does not alter the authors’ adherence to all the PLOS ONE policies on sharing data and materials. MLS-K, A-RFK, JDT, CLW, and DSA contributed to this work while employees of VLST Corporation. These individuals are currently affiliated with other commercial or academic entities. Their current and former affiliations do not alter their adherence to all the PLOS ONE policies on sharing data and materials.

Author Contributions

Conceived and designed the experiments: MLS-K DLB PP RAS DSA TMF CJR. Performed the experiments: MLS-K BWH RLK A-RFK KHL JSM PJO ARP PJS JDLT CLW CJR. Analyzed the data: MLS-K DSA TMF CJR. Wrote the paper: MLS-K TMF CJR.