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Research Article

Generation of Induced Pluripotent Stem Cells from Human Nasal Epithelial Cells Using a Sendai Virus Vector

  • Mizuho Ono,

    Affiliation: Department of Medical Genetics, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan

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  • Yuko Hamada,

    Affiliation: Department of Medical Genetics, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan

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  • Yasue Horiuchi,

    Affiliation: Department of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America

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  • Mami Matsuo-Takasaki,

    Affiliation: Department of Regenerative Medicine and Stem Cell Biology, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan

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  • Yoshimasa Imoto,

    Affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Faculty of Medical Sciences, University of Fukui, Fukui City, Fukui, Japan

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  • Kaishi Satomi,

    Affiliation: Department of Diagnostic Pathology, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan

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  • Tadao Arinami,

    Affiliation: Department of Medical Genetics, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan

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  • Mamoru Hasegawa,

    Affiliation: DNAVEC Research Inc, Tsukuba, Ibaraki, Japan

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  • Tsuyoshi Fujioka,

    Affiliation: Cell Engineering Division, RIKEN BioResource Center, Tsukuba, Ibaraki, Japan

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  • Yukio Nakamura,

    Affiliation: Cell Engineering Division, RIKEN BioResource Center, Tsukuba, Ibaraki, Japan

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  • Emiko Noguchi mail

    enoguchi@md.tsukuba.ac.jp

    Affiliations: Department of Medical Genetics, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan, Japan Science and Technology Agency, Core Research for Evolutional Science and Technology (CREST), Chiyoda, Tokyo, Japan

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  • Published: August 13, 2012
  • DOI: 10.1371/journal.pone.0042855

About the Authors

Mizuho Ono, Yuko Hamada, Tadao Arinami, Emiko Noguchi
Department of Medical Genetics, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan
Yasue Horiuchi
Department of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America
Mami Matsuo-Takasaki
Department of Regenerative Medicine and Stem Cell Biology, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan
Yoshimasa Imoto
Department of Otorhinolaryngology Head and Neck Surgery, Faculty of Medical Sciences, University of Fukui, Fukui City, Fukui, Japan
Kaishi Satomi
Department of Diagnostic Pathology, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan
Mamoru Hasegawa
DNAVEC Research Inc, Tsukuba, Ibaraki, Japan
Tsuyoshi Fujioka, Yukio Nakamura
Cell Engineering Division, RIKEN BioResource Center, Tsukuba, Ibaraki, Japan
Emiko Noguchi
Japan Science and Technology Agency, Core Research for Evolutional Science and Technology (CREST), Chiyoda, Tokyo, Japan

Corresponding Author

Email: enoguchi@md.tsukuba.ac.jp

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: Y. Horiuchi MMT TA EN. Performed the experiments: MO Y. Hamada YI KS TF. Analyzed the data: MO TH EN. Contributed reagents/materials/analysis tools: MMT YN MH. Wrote the paper: MO Y. Hamada MMT EN.