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Research Article

Towards a Pathogenic Escherichia coli Detection Platform Using Multiplex SYBR®Green Real-Time PCR Methods and High Resolution Melting Analysis

  • Dafni-Maria Kagkli,

    Affiliation: European Commission Joint Research Centre, Institute for Health and Consumer Protection, Molecular Biology and Genomics Unit, Ispra (VA), Italy

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  • Silvia Folloni,

    Affiliation: European Commission Joint Research Centre, Institute for Health and Consumer Protection, Molecular Biology and Genomics Unit, Ispra (VA), Italy

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  • Elodie Barbau-Piednoir,

    Affiliation: Scientific Institute of Public Health, Brussels, Belgium

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  • Guy Van den Eede,

    Affiliation: European Commission Joint Research Centre, Institute for Health and Consumer Protection, Molecular Biology and Genomics Unit, Ispra (VA), Italy

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  • Marc Van den Bulcke mail

    Marc.VAN-DEN-BULCKE@ec.europa.eu

    Affiliation: European Commission Joint Research Centre, Institute for Health and Consumer Protection, Molecular Biology and Genomics Unit, Ispra (VA), Italy

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  • Published: June 25, 2012
  • DOI: 10.1371/journal.pone.0039287

About the Authors

Dafni-Maria Kagkli, Silvia Folloni, Guy Van den Eede, Marc Van den Bulcke
European Commission Joint Research Centre, Institute for Health and Consumer Protection, Molecular Biology and Genomics Unit, Ispra (VA), Italy
Elodie Barbau-Piednoir
Scientific Institute of Public Health, Brussels, Belgium

Corresponding Author

Email: Marc.VAN-DEN-BULCKE@ec.europa.eu

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: MVDB DMK. Performed the experiments: DMK SF. Analyzed the data: DMK MVDB. Contributed reagents/materials/analysis tools: EBP. Wrote the paper: DMK MVDB GVDE.