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Research Article

Thermostable DNA Polymerase from a Viral Metagenome Is a Potent RT-PCR Enzyme

  • Michael J. Moser,
  • Robert A. DiFrancesco,
  • Krishne Gowda,
  • Audrey J. Klingele,
  • Darby R. Sugar,
  • Stacy Stocki,
  • David A. Mead,
  • Thomas W. Schoenfeld mail

    tschoenfeld@lucigen.com

    Affiliation: Lucigen Corporation, Middleton, Wisconsin, United States of America

    X
  • Published: June 04, 2012
  • DOI: 10.1371/journal.pone.0038371

About the Authors

Thomas W. Schoenfeld
Lucigen Corporation, Middleton, Wisconsin, United States of America

Corresponding Author

Email: tschoenfeld@lucigen.com

Competing Interests

Michael J. Moser, Krishne Gowda, David A. Mead, and Thomas W. Schoenfeld are employed by Lucigen Corporation. Darby R. Sugar is currently employed by Madison College. Staci Stocki is currently employed by SciLog BioProcessing Systems. Robert A. DiFrancesco is currently employed by Epicentre Technologies. All authors declare no other relevant declarations relating to employment, consultancy, products in development or marketed products. The enzyme described in this report is the subject of a patent application under consideration in the U.S. and other countries (US20080268498, EP1934339). This does not alter the authors' adherence to all the PLoS ONE policies on sharing data and materials.

Author Contributions

Conceived and designed the experiments: MJM TWS DAM. Performed the experiments: MJM RAD DRS AJK SJS TWS. Analyzed the data: MJM RAD TWS. Contributed reagents/materials/analysis tools: KG. Wrote the paper: TWS MJM DAM.