About the Authors

Elizabeth E. Gray

Affiliation Howard Hughes Medical Institute and Department of Microbiology and Immunology, University of California San Francisco (UCSF), San Francisco, California, United States of America

Sherree Friend

Affiliation Amnis Corporation, Seattle, Washington, United States of America

Kazuhiro Suzuki

Current address: Laboratory of Immune Response Dynamics, WPI Immunology Frontier Research Center, Osaka University, Suita, Osaka, Japan

Affiliation Howard Hughes Medical Institute and Department of Microbiology and Immunology, University of California San Francisco (UCSF), San Francisco, California, United States of America

Tri Giang Phan

Affiliations Garvan Institute of Medical Research, Sydney, New South Wales, Australia, St Vincent's Clinical School, Faculty of Medicine, University of New South Wales, Sydney, Australia

Jason G. Cyster

jason.cyster@ucsf.edu

Affiliation Howard Hughes Medical Institute and Department of Microbiology and Immunology, University of California San Francisco (UCSF), San Francisco, California, United States of America

Competing Interests

The authors have read the journal's policy and have the following conflicts: Sherree Friend is an employee of Amnis Corporation, which manufactures ImageStream. This does not alter the authors' adherence to all the PLoS ONE policies on sharing data and materials.

Author Contributions

Conceived and designed the experiments: EEG KS TGP JGC. Performed the experiments: EEG SF KS. Analyzed the data: EEG SF KS TGP JGC. Contributed reagents/materials/analysis tools: SF JGC. Wrote the paper: EEG JGC.