About the Authors

Matthew C. Leach

Matthew.Leach@Newcastle.ac.uk

Affiliation Institute of Neuroscience and Comparative Biology Centre, Newcastle University, Newcastle upon Tyne, United Kingdom

Kristel Klaus

Affiliation Institute of Neuroscience and Comparative Biology Centre, Newcastle University, Newcastle upon Tyne, United Kingdom

Amy L. Miller

Affiliation Institute of Neuroscience and Comparative Biology Centre, Newcastle University, Newcastle upon Tyne, United Kingdom

Maud Scotto di Perrotolo

Affiliation Institute of Neuroscience and Comparative Biology Centre, Newcastle University, Newcastle upon Tyne, United Kingdom

Susana G. Sotocinal

Affiliation Department of Psychology and Alan Edwards Centre for Research on Pain, McGill University, Montreal, Quebec, Canada

Paul A. Flecknell

Affiliation Institute of Neuroscience and Comparative Biology Centre, Newcastle University, Newcastle upon Tyne, United Kingdom

Competing Interests

The authors have read the journal's policy and have the following conflicts. The commercial organizations AFSTAL (the French Laboratory Animal Science Association) and Laboratory Animals Ltd provided financial support for Ms. Scotto in the form of a training scholarship. The commercial organization Charles River UK donated the animals used in the study. This does not alter the authors' adherence to all the PLoS ONE policies on sharing data and materials.

Author Contributions

Conceived and designed the experiments: MCL KK ALM MSP SS PAF. Performed the experiments: MCL ALM MSP. Analyzed the data: MCL. Read, edited and approved the final manuscript: MCL KK ALM MSP SS PAF. Provided the Mouse Grimace Scale guide and gave technical support: SS. Interpreted the results and drafted the manuscript: MCL.