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Research Article

Single Cell Profiling of Circulating Tumor Cells: Transcriptional Heterogeneity and Diversity from Breast Cancer Cell Lines

  • Ashley A. Powell equal contributor,

    equal contributor Contributed equally to this work with: Ashley A. Powell, AmirAli H. Talasaz, Haiyu Zhang

    Affiliation: Department of Surgery, Stanford University School of Medicine, Stanford, California, United States of America

    Current address: Department of Obstetrics and Gynecology, Stanford University School of Medicine, Stanford, California, United States of America

    X
  • AmirAli H. Talasaz equal contributor,

    equal contributor Contributed equally to this work with: Ashley A. Powell, AmirAli H. Talasaz, Haiyu Zhang

    Affiliations: Stanford Genome Technology Center, Stanford University, Palo Alto, California, United States of America, Department of Electrical Engineering, Stanford University, Stanford, California, United States of America

    Current address: Department of Diagnostic Research, Illumina, Inc., Hayward, California, United States of America

    X
  • Haiyu Zhang equal contributor,

    equal contributor Contributed equally to this work with: Ashley A. Powell, AmirAli H. Talasaz, Haiyu Zhang

    Affiliation: Department of Surgery, Stanford University School of Medicine, Stanford, California, United States of America

    X
  • Marc A. Coram,

    Affiliation: Department of Health Research and Policy (Biostatistics), Stanford University School of Medicine, Stanford, California, United States of America

    X
  • Anupama Reddy,

    Affiliation: BioMaPS Institute for Quantitative Biology, Department of Physics, Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, New Jersey, United States of America

    Current address: Novartis Institutes for Biomedical Research, Cambridge, Massachusetts, United States of America

    X
  • Glenn Deng,

    Affiliation: Department of Surgery, Stanford University School of Medicine, Stanford, California, United States of America

    Current address: Cancer Research Institute, College of Medicine, Xiangfan University, Xiangyang, Hubei, China

    X
  • Melinda L. Telli,

    Affiliation: Department of Medicine, Stanford University School of Medicine, Stanford, California, United States of America

    X
  • Ranjana H. Advani,

    Affiliation: Department of Medicine, Stanford University School of Medicine, Stanford, California, United States of America

    X
  • Robert W. Carlson,

    Affiliation: Department of Medicine, Stanford University School of Medicine, Stanford, California, United States of America

    X
  • Joseph A. Mollick,

    Affiliation: Department of Medicine, Stanford University School of Medicine, Stanford, California, United States of America

    X
  • Shruti Sheth,

    Affiliation: Department of Medicine, Stanford University School of Medicine, Stanford, California, United States of America

    X
  • Allison W. Kurian,

    Affiliation: Department of Medicine, Stanford University School of Medicine, Stanford, California, United States of America

    X
  • James M. Ford,

    Affiliation: Department of Medicine, Stanford University School of Medicine, Stanford, California, United States of America

    X
  • Frank E. Stockdale,

    Affiliation: Department of Medicine, Stanford University School of Medicine, Stanford, California, United States of America

    X
  • Stephen R. Quake,

    Affiliation: Howard Hughes Medical Institute, Department of Bioengineering, Stanford University, Stanford, California, United States of America

    X
  • R. Fabian Pease,

    Affiliation: Department of Electrical Engineering, Stanford University, Stanford, California, United States of America

    X
  • Michael N. Mindrinos,

    Affiliation: Stanford Genome Technology Center, Stanford University, Palo Alto, California, United States of America

    X
  • Gyan Bhanot,

    Affiliations: BioMaPS Institute for Quantitative Biology, Department of Physics, Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, New Jersey, United States of America, Cancer Institute of New Jersey, New Brunswick, New Jersey, United States of America, Simons Center for Systems Biology, Institute for Advanced Study, Princeton, New Jersey, United States of America

    X
  • Shanaz H. Dairkee mail,

    ssj@stanford.edu (SSJ); dbowe@stanford.edu (RWD); dairkes@cpmcri.org (SHD)

    Affiliations: Department of Surgery, Stanford University School of Medicine, Stanford, California, United States of America, California Pacific Medical Center Research Institute, San Francisco, California, United States of America

    X
  • Ronald W. Davis mail,

    ssj@stanford.edu (SSJ); dbowe@stanford.edu (RWD); dairkes@cpmcri.org (SHD)

    Affiliation: Stanford Genome Technology Center, Stanford University, Palo Alto, California, United States of America

    X
  • Stefanie S. Jeffrey mail

    ssj@stanford.edu (SSJ); dbowe@stanford.edu (RWD); dairkes@cpmcri.org (SHD)

    Affiliation: Department of Surgery, Stanford University School of Medicine, Stanford, California, United States of America

    X
  • Published: May 07, 2012
  • DOI: 10.1371/journal.pone.0033788

About the Authors

Ashley A. Powell, Haiyu Zhang, Glenn Deng, Shanaz H. Dairkee, Stefanie S. Jeffrey
Department of Surgery, Stanford University School of Medicine, Stanford, California, United States of America
AmirAli H. Talasaz, Michael N. Mindrinos, Ronald W. Davis
Stanford Genome Technology Center, Stanford University, Palo Alto, California, United States of America
AmirAli H. Talasaz, R. Fabian Pease
Department of Electrical Engineering, Stanford University, Stanford, California, United States of America
Marc A. Coram
Department of Health Research and Policy (Biostatistics), Stanford University School of Medicine, Stanford, California, United States of America
Anupama Reddy, Gyan Bhanot
BioMaPS Institute for Quantitative Biology, Department of Physics, Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, New Jersey, United States of America
Melinda L. Telli, Ranjana H. Advani, Robert W. Carlson, Joseph A. Mollick, Shruti Sheth, Allison W. Kurian, James M. Ford, Frank E. Stockdale
Department of Medicine, Stanford University School of Medicine, Stanford, California, United States of America
Stephen R. Quake
Howard Hughes Medical Institute, Department of Bioengineering, Stanford University, Stanford, California, United States of America
Gyan Bhanot
Cancer Institute of New Jersey, New Brunswick, New Jersey, United States of America
Gyan Bhanot
Simons Center for Systems Biology, Institute for Advanced Study, Princeton, New Jersey, United States of America
Shanaz H. Dairkee
California Pacific Medical Center Research Institute, San Francisco, California, United States of America

Corresponding Authors

Competing Interests

Dr. Stefanie Jeffrey, Dr. Ashley Powell, Dr. AmirAli Talasaz, Dr. Michael Mindrinos, Dr. Fabian Pease, and Dr. Ronald Davis are inventors of the MagSweeper technology used in this study. Stanford University has licensed this technology to Illumina, Inc., and receives licensing royalties. Dr. Jeffrey has donated her royalties to support student programs at The Jackson Laboratory, a nonprofit biomedical research institution. Dr. AmirAli Talasaz is currently employed by Illumina. Dr. Stephen Quake is a co-founder of Fluidigm Corporation and member of its board of directors and scientific advisory board. Fluidigm chips were used in this study. This does not alter the authors’ adherence to all the PLoS ONE policies on sharing data and materials.

Author Contributions

Conceived and designed the experiments: SSJ AAP AHT HZ SHD MNM SRQ RFP RWD. Performed the experiments: AAP HZ GD. Analyzed the data: MAC AR GB HZ SSJ SHD. Contributed reagents/materials/analysis tools: MLT RHA RWC JAM SS AWK JMF FES SRQ. Wrote the paper: SSJ SHD AAP AHT MNM HZ. Invented the MagSweeper: AHT AAP MNM RFP RWD SSJ. Optimized MagSweeper configuration and performance: AAP AHT.