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Research Article

Observation of Live Ticks (Haemaphysalis flava) by Scanning Electron Microscopy under High Vacuum Pressure

  • Yasuhito Ishigaki,

    Affiliation: Medical Research Institute, Kanazawa Medical University, Uchinada-machi, Kahoku-gun, Japan

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  • Yuka Nakamura,

    Affiliation: Medical Research Institute, Kanazawa Medical University, Uchinada-machi, Kahoku-gun, Japan

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  • Yosaburo Oikawa,

    Affiliation: Department of Medical Zoology, Kanazawa Medical University, Uchinada-machi, Kahoku-gun, Japan

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  • Yasuhiro Yano,

    Affiliation: Department of Pathological Sciences, Faculty of Medical Sciences, University of Fukui, Matsuoka Fukui, Japan

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  • Susumu Kuwabata,

    Affiliations: Department of Applied Chemistry, Graduate School of Engineering, Osaka University, Suita, Osaka, Japan, Japan Science and Technology Agency (JST), Core Research for Evolutional Science and Technology (CREST), Kawaguchi, Saitama, Japan

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  • Hideaki Nakagawa,

    Affiliation: Medical Research Institute, Kanazawa Medical University, Uchinada-machi, Kahoku-gun, Japan

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  • Naohisa Tomosugi mail,

    tomosugi@kanazawa-med.ac.jp

    Affiliation: Medical Research Institute, Kanazawa Medical University, Uchinada-machi, Kahoku-gun, Japan

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  • Tsutomu Takegami

    Affiliation: Medical Research Institute, Kanazawa Medical University, Uchinada-machi, Kahoku-gun, Japan

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  • Published: March 14, 2012
  • DOI: 10.1371/journal.pone.0032676

About the Authors

Yasuhito Ishigaki, Yuka Nakamura, Hideaki Nakagawa, Naohisa Tomosugi, Tsutomu Takegami
Medical Research Institute, Kanazawa Medical University, Uchinada-machi, Kahoku-gun, Japan
Yosaburo Oikawa
Department of Medical Zoology, Kanazawa Medical University, Uchinada-machi, Kahoku-gun, Japan
Yasuhiro Yano
Department of Pathological Sciences, Faculty of Medical Sciences, University of Fukui, Matsuoka Fukui, Japan
Susumu Kuwabata
Department of Applied Chemistry, Graduate School of Engineering, Osaka University, Suita, Osaka, Japan
Susumu Kuwabata
Japan Science and Technology Agency (JST), Core Research for Evolutional Science and Technology (CREST), Kawaguchi, Saitama, Japan

Corresponding Author

Email: tomosugi@kanazawa-med.ac.jp

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: YI YN NT. Performed the experiments: YI YN YO YY NT TT. Analyzed the data: HN. Contributed reagents/materials/analysis tools: SK. Wrote the paper: YI NT.