About the Authors
- Robert T. Schinzel
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Affiliations Department of Stem Cell and Regenerative Biology, Harvard Stem Cell Institute, Harvard University, Cambridge, Massachusetts, United States of America, Center for Regenerative Medicine, Massachusetts General Hospital, Boston, Massachusetts, United States of America, Cardiovascular Research Center, Massachusetts General Hospital, Boston, Massachusetts, United States of America, Institut für Biologie - Mikrobiologie, Fachbereich Biologie, Chemie, Pharmazie, Freie Universität Berlin, Berlin, Germany
- Tim Ahfeldt
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Affiliations Department of Stem Cell and Regenerative Biology, Harvard Stem Cell Institute, Harvard University, Cambridge, Massachusetts, United States of America, Center for Regenerative Medicine, Massachusetts General Hospital, Boston, Massachusetts, United States of America, Cardiovascular Research Center, Massachusetts General Hospital, Boston, Massachusetts, United States of America
- Frank H. Lau
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Affiliation Center for Regenerative Medicine, Massachusetts General Hospital, Boston, Massachusetts, United States of America
- Youn-Kyoung Lee
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Affiliations Department of Stem Cell and Regenerative Biology, Harvard Stem Cell Institute, Harvard University, Cambridge, Massachusetts, United States of America, Center for Regenerative Medicine, Massachusetts General Hospital, Boston, Massachusetts, United States of America, Cardiovascular Research Center, Massachusetts General Hospital, Boston, Massachusetts, United States of America
- Alicia Cowley
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Affiliations Department of Stem Cell and Regenerative Biology, Harvard Stem Cell Institute, Harvard University, Cambridge, Massachusetts, United States of America, Center for Regenerative Medicine, Massachusetts General Hospital, Boston, Massachusetts, United States of America, Harvard College, Harvard University, Cambridge, Massachusetts, United States of America
- Tony Shen
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Affiliations Department of Stem Cell and Regenerative Biology, Harvard Stem Cell Institute, Harvard University, Cambridge, Massachusetts, United States of America, Center for Regenerative Medicine, Massachusetts General Hospital, Boston, Massachusetts, United States of America, Harvard College, Harvard University, Cambridge, Massachusetts, United States of America
- Derek Peters
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Affiliations Department of Stem Cell and Regenerative Biology, Harvard Stem Cell Institute, Harvard University, Cambridge, Massachusetts, United States of America, Center for Regenerative Medicine, Massachusetts General Hospital, Boston, Massachusetts, United States of America, Cardiovascular Research Center, Massachusetts General Hospital, Boston, Massachusetts, United States of America
- David H. Lum
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Affiliations Huntsman Cancer Institute, University of Utah, Salt Lake City, Utah, United States of America, Department of Oncological Sciences, University of Utah, Salt Lake City, Utah, United States of America
- Chad A. Cowan
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* E-mail: ccowan@fas.harvard.edu
Affiliations Department of Stem Cell and Regenerative Biology, Harvard Stem Cell Institute, Harvard University, Cambridge, Massachusetts, United States of America, Center for Regenerative Medicine, Massachusetts General Hospital, Boston, Massachusetts, United States of America, Cardiovascular Research Center, Massachusetts General Hospital, Boston, Massachusetts, United States of America
Competing Interests
The authors have declared that no competing interests exist.
Author Contributions
Conceived and designed the experiments: RTS TA CAC. Performed the experiments: RTS TA YKL FHL AC TS DP. Analyzed the data: RTS CAC. Contributed reagents/materials/analysis tools: RTS. Wrote the paper: RTS TA CAC. Lenti-Virus transduction, Non-pluripotent cell abundance and size distribution: TA. Teratoma formation assay: FHL. Lenti-viral transduction efficiency: YKL. Immuno-staining pluripotency, QPCR pluripotency: AC. Size distribution, Lenti viral transduction setup figure 2a: TS. Elctroporation efficiency: DP. Plasmid design and vectors: DHL. Fluorescence pictures, Lenti-viral transduction: TS. qPCR pluripotency, immunofluorescence pictures pluripotency: AC. Determination of the Lenti viral titer: YKL. Evaluation of Lenit-viral transduction and non-pluripotent cell abundance FACS data: TA. Efficiency of electroporation: DP. For GFP-reporter constructs: DHL.