About the Authors

Matthew M. Cousins

Affiliation Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America

Oliver Laeyendecker

Affiliations Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America, Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Baltimore, Maryland, United States of America

Geetha Beauchamp

Affiliation Statistical Center for HIV/AIDS Research and Prevention, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America

Ronald Brookmeyer

Affiliation Department of Biostatistics, University of California Los Angeles, Los Angeles, California, United States of America

William I. Towler

Affiliation Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America

Sarah E. Hudelson

Affiliation Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America

Leila Khaki

Affiliation Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America

Beryl Koblin

Affiliation Laboratory of Infectious Disease Prevention, New York Blood Center, New York, New York, United States of America

Margaret Chesney

Affiliation University of California San Francisco, San Francisco, California, United States of America

Richard D. Moore

Affiliation Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America

Gabor D. Kelen

Affiliation Department of Emergency Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America

Thomas Coates

Affiliation Program in Global Health, University of California Los Angeles, Los Angeles, California, United States of America

Connie Celum

Affiliation Department of Medicine, University of Washington, Seattle, Washington, United States of America

Susan P. Buchbinder

Affiliations University of California San Francisco, San Francisco, California, United States of America, San Francisco Department of Public Health, San Francisco, California, United States of America

George R. Seage III

Affiliation Department of Epidemiology, Harvard School of Public Health, Boston, Massachusetts, United States of America

Thomas C. Quinn

Affiliations Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America, Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Baltimore, Maryland, United States of America

Deborah Donnell

Affiliation Statistical Center for HIV/AIDS Research and Prevention, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America

Susan H. Eshleman

seshlem@jhmi.edu

Affiliation Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America

Competing Interests

S.H.E. has given presentations at meetings sponsored by Abbott Diagnostics (distributor of the ViroSeq HIV Genotyping System) and has collaborated with Celera (manufacturer of the ViroSeq HIV Genotyping System) and Abbott Diagnostics on evaluation of HIV-related assays. S.H.E. and W.T. are co-inventors of the HRM diversity assay, and Johns Hopkins University has filed a patent application for this assay with the US-Patent and Trademark Office under the title ‘Use of a high-resolution melting assay to measure genetic diversity’. The inventors may receive royalty payments if the patent is awarded and licensed. This does not alter our adherence to all the PLoS ONE policies on sharing data and materials.

Author Contributions

Conceived and designed the experiments: MMC OL WIT SHE. Performed the experiments: MMC WIT SEH LK. Analyzed the data: MMC SHE GB RB DD. Contributed reagents/materials/analysis tools: BK MC RDM GDK TC CC SPB GRS. Wrote the paper: MMC RB DD SHE. All authors contributed to manuscript preparation: MMC OL GB RB WIT SEH LK BK MC RDM GDK TC CC SPB GRS TCQ DD SHE.