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Research Article

Acute Drug Treatment in the Early C. elegans Embryo

  • Ana Carvalho equal contributor,

    equal contributor Contributed equally to this work with: Ana Carvalho, Sara K. Olson, Edgar Gutierrez

    Affiliation: Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, California, United States of America

    X
  • Sara K. Olson equal contributor,

    equal contributor Contributed equally to this work with: Ana Carvalho, Sara K. Olson, Edgar Gutierrez

    Affiliation: Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, California, United States of America

    X
  • Edgar Gutierrez equal contributor,

    equal contributor Contributed equally to this work with: Ana Carvalho, Sara K. Olson, Edgar Gutierrez

    Affiliation: Department of Physics, University of California San Diego, La Jolla, California, United States of America

    X
  • Kelly Zhang,

    Affiliation: Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, California, United States of America

    X
  • Lisa B. Noble,

    Affiliation: Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, California, United States of America

    X
  • Esther Zanin,

    Affiliation: Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, California, United States of America

    X
  • Arshad Desai,

    Affiliation: Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, California, United States of America

    X
  • Alex Groisman mail,

    koegema@ucsd.edu (KO); agroisman@ucsd.edu (AG)

    Affiliation: Department of Physics, University of California San Diego, La Jolla, California, United States of America

    X
  • Karen Oegema mail

    koegema@ucsd.edu (KO); agroisman@ucsd.edu (AG)

    Affiliation: Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, California, United States of America

    X
  • Published: September 14, 2011
  • DOI: 10.1371/journal.pone.0024656

About the Authors

Ana Carvalho, Sara K. Olson, Kelly Zhang, Lisa B. Noble, Esther Zanin, Arshad Desai, Karen Oegema
Ludwig Institute for Cancer Research, Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, California, United States of America
Edgar Gutierrez, Alex Groisman
Department of Physics, University of California San Diego, La Jolla, California, United States of America

Corresponding Authors

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: AC SO EG AG KO. Performed the experiments: AC SO EG KZ LN EZ. Analyzed the data: AC SO EG AG KO. Wrote the paper: AC SO AD AG KO. Performed screen to identify permeability genes: SO KZ LN. Optimized perm-1 RNAi: AC SO EZ. Performed drug delivery experiments: AC. Designed microdevice: EG AG. Built microdevices: EG.