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Research Article

A Selectable and Excisable Marker System for the Rapid Creation of Recombinant Poxviruses

  • Julia L. Rintoul equal contributor,

    equal contributor Contributed equally to this work with: Julia L. Rintoul, Jiahu Wang

    Affiliations: Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, Canada, Centre for Cancer Therapeutics, Ottawa Hospital Research Institute, Ottawa, Canada

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  • Jiahu Wang equal contributor,

    equal contributor Contributed equally to this work with: Julia L. Rintoul, Jiahu Wang

    Affiliation: Centre for Cancer Therapeutics, Ottawa Hospital Research Institute, Ottawa, Canada

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  • Don B. Gammon,

    Affiliation: Department of Medical Microbiology and Immunology, Faculty of Medicine and Dentistry, Li Ka Shing Institute of Virology, University of Alberta, Edmonton, Canada

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  • Nicholas J. van Buuren,

    Affiliation: Department of Medical Microbiology and Immunology, Faculty of Medicine and Dentistry, Li Ka Shing Institute of Virology, University of Alberta, Edmonton, Canada

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  • Kenneth Garson,

    Affiliation: Centre for Cancer Therapeutics, Ottawa Hospital Research Institute, Ottawa, Canada

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  • Karen Jardine,

    Affiliation: Centre for Cancer Therapeutics, Ottawa Hospital Research Institute, Ottawa, Canada

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  • Michele Barry,

    Affiliation: Department of Medical Microbiology and Immunology, Faculty of Medicine and Dentistry, Li Ka Shing Institute of Virology, University of Alberta, Edmonton, Canada

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  • David H. Evans,

    Affiliation: Department of Medical Microbiology and Immunology, Faculty of Medicine and Dentistry, Li Ka Shing Institute of Virology, University of Alberta, Edmonton, Canada

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  • John C. Bell mail

    jbell@ohri.ca

    Affiliations: Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, Canada, Centre for Cancer Therapeutics, Ottawa Hospital Research Institute, Ottawa, Canada

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  • Published: September 08, 2011
  • DOI: 10.1371/journal.pone.0024643

About the Authors

Julia L. Rintoul, John C. Bell
Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, Canada
Julia L. Rintoul, Jiahu Wang, Kenneth Garson, Karen Jardine, John C. Bell
Centre for Cancer Therapeutics, Ottawa Hospital Research Institute, Ottawa, Canada
Don B. Gammon, Nicholas J. van Buuren, Michele Barry, David H. Evans
Department of Medical Microbiology and Immunology, Faculty of Medicine and Dentistry, Li Ka Shing Institute of Virology, University of Alberta, Edmonton, Canada

Corresponding Author

Email: jbell@ohri.ca

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: JLR JW DBG NJVB KG KJ MB DHE JCB. Performed the experiments: JLR JW DBG NJVB KG. Analyzed the data: JLR JW DBG NJVB KG KJ MB DHE JCB. Contributed reagents/materials/analysis tools: JLR JW DBG NJVB KG KJ MB DHE JCB. Wrote the paper: JLR JW JCB.