The Nucleotide Exchange Factor Ric-8A Is a Chaperone for the Conformationally Dynamic Nucleotide-Free State of Gαi1

Celestine J. Thomas; Klára Briknarová; Jonathan K. Hilmer; Navid Movahed; Brian Bothner; John P. Sumida; Gregory G. Tall; Stephen R. Sprang

doi:10.1371/journal.pone.0023197

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Peer-reviewed

Research Article

The Nucleotide Exchange Factor Ric-8A Is a Chaperone for the Conformationally Dynamic Nucleotide-Free State of Gαi1

Celestine J. Thomas,

Affiliations Center for Biomolecular Structure and Dynamics, The University of Montana, Missoula, Montana, United States of America, Division of Biological Science, The University of Montana, Missoula, Montana, United States of America
⨯
Klára Briknarová,

Affiliations Center for Biomolecular Structure and Dynamics, The University of Montana, Missoula, Montana, United States of America, Department of Chemistry and Biochemistry, The University of Montana, Missoula, Montana, United States of America
⨯
Jonathan K. Hilmer,

Affiliation Proteomics and Mass Spectrometry Facility, Department of Chemistry and Biochemistry, Montana State University, Bozeman, Montana, United States of America
⨯
Navid Movahed,

Affiliation Proteomics and Mass Spectrometry Facility, Department of Chemistry and Biochemistry, Montana State University, Bozeman, Montana, United States of America
⨯
Brian Bothner,

Affiliation Proteomics and Mass Spectrometry Facility, Department of Chemistry and Biochemistry, Montana State University, Bozeman, Montana, United States of America
⨯
John P. Sumida,

Affiliation Bioanalytical Pharmacy Core, University of Washington, Seattle, Washington, United States of America
⨯
Gregory G. Tall,

Affiliation Department of Pharmacology and Physiology, School of Medicine and Dentistry, University of Rochester Medical Center, Rochester, New York, United States of America
⨯
Stephen R. Sprang

* E-mail: Stephen.sprang@umontana.edu

Affiliations Center for Biomolecular Structure and Dynamics, The University of Montana, Missoula, Montana, United States of America, Division of Biological Science, The University of Montana, Missoula, Montana, United States of America
⨯

The Nucleotide Exchange Factor Ric-8A Is a Chaperone for the Conformationally Dynamic Nucleotide-Free State of Gαi1

Celestine J. Thomas,
Klára Briknarová,
Jonathan K. Hilmer,
Navid Movahed,
Brian Bothner,
John P. Sumida,
Gregory G. Tall,
Stephen R. Sprang

Published: August 11, 2011
https://doi.org/10.1371/journal.pone.0023197

About the Authors

Celestine J. Thomas

Affiliations Center for Biomolecular Structure and Dynamics, The University of Montana, Missoula, Montana, United States of America, Division of Biological Science, The University of Montana, Missoula, Montana, United States of America

Klára Briknarová

Affiliations Center for Biomolecular Structure and Dynamics, The University of Montana, Missoula, Montana, United States of America, Department of Chemistry and Biochemistry, The University of Montana, Missoula, Montana, United States of America

Jonathan K. Hilmer

Affiliation Proteomics and Mass Spectrometry Facility, Department of Chemistry and Biochemistry, Montana State University, Bozeman, Montana, United States of America

Navid Movahed

Affiliation Proteomics and Mass Spectrometry Facility, Department of Chemistry and Biochemistry, Montana State University, Bozeman, Montana, United States of America

Brian Bothner

Affiliation Proteomics and Mass Spectrometry Facility, Department of Chemistry and Biochemistry, Montana State University, Bozeman, Montana, United States of America

John P. Sumida

Affiliation Bioanalytical Pharmacy Core, University of Washington, Seattle, Washington, United States of America

Gregory G. Tall

Affiliation Department of Pharmacology and Physiology, School of Medicine and Dentistry, University of Rochester Medical Center, Rochester, New York, United States of America

Stephen R. Sprang

* E-mail: Stephen.sprang@umontana.edu

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: SRS CJT JPS BB KB JH GGT. Performed the experiments: CJT JPS KB NM JH GGT. Analyzed the data: SRS KB CJT JPS JH GGT . Contributed reagents/materials/analysis tools: KB JPS JH BB. Wrote the paper: SRS CJT JPS JH.

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Nucleotides
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Guanine nucleotide exchange factors
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Membrane proteins
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NMR spectroscopy
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G protein coupled receptors
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Proteases
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Serine proteases
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Protein structure
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