# wombat urls ß PLOS ONE: Automated Microinjection of Recombinant BCL-X into Mouse Zygotes Enhances Embryo Development
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Research Article

Automated Microinjection of Recombinant BCL-X into Mouse Zygotes Enhances Embryo Development

  • Xinyu Liu equal contributor,

    equal contributor Contributed equally to this work with: Xinyu Liu, Roxanne Fernandes

    Affiliation: Department of Mechanical and Industrial Engineering and Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario, Canada

    Current address: Department of Mechanical Engineering, McGill University, Montreal, Quebec, Canada

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  • Roxanne Fernandes equal contributor,

    equal contributor Contributed equally to this work with: Xinyu Liu, Roxanne Fernandes

    Affiliation: Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada

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  • Marina Gertsenstein,

    Affiliation: Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada

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  • Alagammal Perumalsamy,

    Affiliation: Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada

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  • Ingrid Lai,

    Affiliation: Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada

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  • Maggie Chi,

    Affiliation: Department of Obstetrics and Gynecology, Washington University in St. Louis, St. Louis, Missouri, United States of America

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  • Kelle H. Moley,

    Affiliation: Department of Obstetrics and Gynecology, Washington University in St. Louis, St. Louis, Missouri, United States of America

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  • Ellen Greenblatt,

    Affiliation: Department of Obstetrics and Gynecology, University of Toronto, Toronto, Ontario, Canada

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  • Igor Jurisica,

    Affiliation: Departments of Computer Science and Medical Biophysics, Ontario Cancer Institute and the Campbell Family Institute for Cancer Research, University Health Network, University of Toronto, Toronto, Ontario, Canada

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  • Robert F. Casper,

    Affiliations: Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada, Department of Obstetrics and Gynecology, University of Toronto, Toronto, Ontario, Canada

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  • Yu Sun mail,

    sun@mie.utoronto.ca (YS); jurisicova@lunenfeld.ca (AJ)

    Affiliation: Department of Mechanical and Industrial Engineering and Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario, Canada

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  • Andrea Jurisicova mail

    sun@mie.utoronto.ca (YS); jurisicova@lunenfeld.ca (AJ)

    Affiliations: Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada, Department of Obstetrics and Gynecology, University of Toronto, Toronto, Ontario, Canada

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  • Published: July 20, 2011
  • DOI: 10.1371/journal.pone.0021687

About the Authors

Xinyu Liu, Yu Sun
Department of Mechanical and Industrial Engineering and Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario, Canada
Roxanne Fernandes, Marina Gertsenstein, Alagammal Perumalsamy, Ingrid Lai, Robert F. Casper, Andrea Jurisicova
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
Maggie Chi, Kelle H. Moley
Department of Obstetrics and Gynecology, Washington University in St. Louis, St. Louis, Missouri, United States of America
Ellen Greenblatt, Robert F. Casper, Andrea Jurisicova
Department of Obstetrics and Gynecology, University of Toronto, Toronto, Ontario, Canada
Igor Jurisica
Departments of Computer Science and Medical Biophysics, Ontario Cancer Institute and the Campbell Family Institute for Cancer Research, University Health Network, University of Toronto, Toronto, Ontario, Canada

Corresponding Authors

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: RFC YS AJ. Performed the experiments: XYL RF AP IL MG MC AJ. Analyzed the data: XYL RF AP IJ KM YS AJ. Contributed reagents/materials/analysis tools: EG KHM IJ. Wrote the paper: XYL YS AJ. Developed the robotic microinjection system and performed robotic microinjection experiments: XYL YS. Performed embryo culture, scoring, and data analysis: AJ. Performed manual embryo microinjection: MG. Oversaw human sample collection: EG.