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Research Article

Linkage Mapping and Comparative Genomics Using Next-Generation RAD Sequencing of a Non-Model Organism

  • Simon W. Baxter equal contributor mail,

    equal contributor Contributed equally to this work with: Simon W. Baxter, John W. Davey

    swb29@cam.ac.uk

    Affiliation: Department of Zoology, University of Cambridge, Cambridge, United Kingdom

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  • John W. Davey equal contributor,

    equal contributor Contributed equally to this work with: Simon W. Baxter, John W. Davey

    Affiliation: Institute of Evolutionary Biology, University of Edinburgh, Edinburgh, United Kingdom

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  • J. Spencer Johnston,

    Affiliation: Department of Entomology, Texas A&M University, College Station, Texas, United States of America

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  • Anthony M. Shelton,

    Affiliation: Department of Entomology, Cornell University, New York State Agricultural Experiment Station, Geneva, New York, United States of America

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  • David G. Heckel,

    Affiliation: Department of Entomology, Max Planck Institute for Chemical Ecology, Jena, Germany

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  • Chris D. Jiggins,

    Affiliation: Department of Zoology, University of Cambridge, Cambridge, United Kingdom

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  • Mark L. Blaxter

    Affiliations: Institute of Evolutionary Biology, University of Edinburgh, Edinburgh, United Kingdom, The GenePool Genomics Facility, Institute of Evolutionary Biology, University of Edinburgh, Edinburgh, United Kingdom

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  • Published: April 26, 2011
  • DOI: 10.1371/journal.pone.0019315

About the Authors

Simon W. Baxter, Chris D. Jiggins
Department of Zoology, University of Cambridge, Cambridge, United Kingdom
John W. Davey, Mark L. Blaxter
Institute of Evolutionary Biology, University of Edinburgh, Edinburgh, United Kingdom
J. Spencer Johnston
Department of Entomology, Texas A&M University, College Station, Texas, United States of America
Anthony M. Shelton
Department of Entomology, Cornell University, New York State Agricultural Experiment Station, Geneva, New York, United States of America
David G. Heckel
Department of Entomology, Max Planck Institute for Chemical Ecology, Jena, Germany
Mark L. Blaxter
The GenePool Genomics Facility, Institute of Evolutionary Biology, University of Edinburgh, Edinburgh, United Kingdom

Corresponding Author

Email: swb29@cam.ac.uk

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: SWB JWD CDJ MLB. Performed the experiments: SWB JSJ MLB. Analyzed the data: SWB JWD. Contributed reagents/materials/analysis tools: AMS DGH MLB. Wrote the paper: SWB DWJ DGH CDJ MLB. Wrote custom Perl scripts for data analysis: JWD.