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Research Article

Drop-on-Demand Single Cell Isolation and Total RNA Analysis

  • Sangjun Moon,

    Affiliation: Demirci Bio-Acoustic-MEMS in Medicine (BAMM) Laboratory, Center for Bioengineering, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, United States of America

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  • Yun-Gon Kim,

    Affiliation: Demirci Bio-Acoustic-MEMS in Medicine (BAMM) Laboratory, Center for Bioengineering, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, United States of America

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  • Lingsheng Dong,

    Affiliation: Renal Division, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, United States of America

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  • Michael Lombardi,

    Affiliation: Renal Division, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, United States of America

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  • Edward Haeggstrom,

    Affiliation: Electronics Research Laboratory, Department of Physics, University of Helsinki, Helsinki, Finland

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  • Roderick V. Jensen,

    Affiliation: Department of Biological Sciences, Virginia Tech, Blacksburg, Virginia, United States of America

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  • Li-Li Hsiao mail,

    udemirci@rics.bwh.harvard.edu (UD); lhsiao@partners.org (L-LH)

    Affiliation: Renal Division, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, United States of America

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  • Utkan Demirci mail

    udemirci@rics.bwh.harvard.edu (UD); lhsiao@partners.org (L-LH)

    Affiliations: Demirci Bio-Acoustic-MEMS in Medicine (BAMM) Laboratory, Center for Bioengineering, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, United States of America, Renal Division, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, United States of America, Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America

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  • Published: March 11, 2011
  • DOI: 10.1371/journal.pone.0017455

About the Authors

Sangjun Moon, Yun-Gon Kim, Utkan Demirci
Demirci Bio-Acoustic-MEMS in Medicine (BAMM) Laboratory, Center for Bioengineering, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, United States of America
Lingsheng Dong, Michael Lombardi, Li-Li Hsiao, Utkan Demirci
Renal Division, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, United States of America
Edward Haeggstrom
Electronics Research Laboratory, Department of Physics, University of Helsinki, Helsinki, Finland
Roderick V. Jensen
Department of Biological Sciences, Virginia Tech, Blacksburg, Virginia, United States of America
Utkan Demirci
Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America

Corresponding Authors

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: SM Y-GK ML L-LH UD. Performed the experiments: SM ML. Analyzed the data: SM Y-GK LD ML EH RJ L-LH UD. Contributed reagents/materials/analysis tools: SM ML. Wrote the paper: SM Y-GK LD ML EH RJ L-LH UD.