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Research Article

Profiling the Dead: Generating Microsatellite Data from Fossil Bones of Extinct Megafauna—Protocols, Problems, and Prospects

  • Morten E. Allentoft mail,

    morten.allentoft@gmail.com (MEA); m.bunce@murdoch.edu.au (MB)

    Affiliations: Ancient DNA Laboratory, School of Biological Sciences and Biotechnology, Murdoch University, Perth, Western Australia, Australia, School of Biological Sciences, University of Canterbury, Christchurch, New Zealand

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  • Charlotte Oskam,

    Affiliation: Ancient DNA Laboratory, School of Biological Sciences and Biotechnology, Murdoch University, Perth, Western Australia, Australia

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  • Jayne Houston,

    Affiliation: Ancient DNA Laboratory, School of Biological Sciences and Biotechnology, Murdoch University, Perth, Western Australia, Australia

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  • Marie L. Hale,

    Affiliation: School of Biological Sciences, University of Canterbury, Christchurch, New Zealand

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  • M. Thomas P Gilbert,

    Affiliation: Centre for GeoGenetics, Natural History Museum of Denmark, Copenhagen, Denmark

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  • Morten Rasmussen,

    Affiliation: Centre for GeoGenetics, Natural History Museum of Denmark, Copenhagen, Denmark

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  • Peter Spencer,

    Affiliation: Wildlife Identification Laboratory, School of Biological Sciences and Biotechnology, Murdoch University, Perth, Western Australia, Australia

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  • Christopher Jacomb,

    Affiliation: Southern Pacific Archaeological Research, Department of Anthropology, University of Otago, Dunedin, New Zealand

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  • Eske Willerslev,

    Affiliation: Centre for GeoGenetics, Natural History Museum of Denmark, Copenhagen, Denmark

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  • Richard N. Holdaway,

    Affiliations: School of Biological Sciences, University of Canterbury, Christchurch, New Zealand, Palaecol Research Ltd, Christchurch, New Zealand

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  • Michael Bunce mail

    morten.allentoft@gmail.com (MEA); m.bunce@murdoch.edu.au (MB)

    Affiliation: Ancient DNA Laboratory, School of Biological Sciences and Biotechnology, Murdoch University, Perth, Western Australia, Australia

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  • Published: January 31, 2011
  • DOI: 10.1371/journal.pone.0016670
  • Featured in PLOS Collections

About the Authors

Morten E. Allentoft, Charlotte Oskam, Jayne Houston, Michael Bunce
Ancient DNA Laboratory, School of Biological Sciences and Biotechnology, Murdoch University, Perth, Western Australia, Australia
Morten E. Allentoft, Marie L. Hale, Richard N. Holdaway
School of Biological Sciences, University of Canterbury, Christchurch, New Zealand
M. Thomas P Gilbert, Morten Rasmussen, Eske Willerslev
Centre for GeoGenetics, Natural History Museum of Denmark, Copenhagen, Denmark
Peter Spencer
Wildlife Identification Laboratory, School of Biological Sciences and Biotechnology, Murdoch University, Perth, Western Australia, Australia
Christopher Jacomb
Southern Pacific Archaeological Research, Department of Anthropology, University of Otago, Dunedin, New Zealand
Richard N. Holdaway
Palaecol Research Ltd, Christchurch, New Zealand

Corresponding Authors

Competing Interests

The authors have declared that no competing interests exist. Palaecol Research Ltd is wholly owned by Richard N Holdaway. It is a requirement of the Marsden Fund of the Royal Society of New Zealand (analogous to NSF etc) when disbursing contestible research funding, that the funds be directed to a non-personal entity. That entity in this instance is the single-person limited liability company, Palaecol Research Ltd, through which the research grant was disbursed. There are no patents, products in development or marketed products to declare. There are therefore no encumbrances on sharing data and materials, as detailed online in the guide for authors.

Author Contributions

Conceived and designed the experiments: MEA MB CO RNH. Performed the experiments: MEA MB CO JH MTPG MR. Analyzed the data: MEA MB. Contributed reagents/materials/analysis tools: MEA MB CO JH RNH MH MTPG MR EW PS CJ. Wrote the paper: MEA MB.