Translational Up-Regulation and High-Level Protein Expression from Plasmid Vectors by mTOR Activation via Different Pathways in PC3 and 293T Cells

Prashanthi Karyala; Nima D. Namsa; Durga Rao Chilakalapudi

doi:10.1371/journal.pone.0014408

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Open Access

Peer-reviewed

Research Article

Translational Up-Regulation and High-Level Protein Expression from Plasmid Vectors by mTOR Activation via Different Pathways in PC3 and 293T Cells

Prashanthi Karyala,

Affiliation Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, India
⨯
Nima D. Namsa,

Affiliation Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, India
⨯
Durga Rao Chilakalapudi

* E-mail: cdr@mcbl.iisc.ernet.in

Affiliation Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, India
⨯

Translational Up-Regulation and High-Level Protein Expression from Plasmid Vectors by mTOR Activation via Different Pathways in PC3 and 293T Cells

Prashanthi Karyala,
Nima D. Namsa,
Durga Rao Chilakalapudi

Published: December 28, 2010
https://doi.org/10.1371/journal.pone.0014408

About the Authors

Prashanthi Karyala

Affiliation Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, India

Nima D. Namsa

Affiliation Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, India

Durga Rao Chilakalapudi

* E-mail: cdr@mcbl.iisc.ernet.in

Affiliation Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, India

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: DRC. Performed the experiments: PK NDN DRC. Analyzed the data: PK DRC. Contributed reagents/materials/analysis tools: DRC. Wrote the paper: PK DRC.

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293T cells
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Transfection
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Phosphorylation
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Protein expression
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Protein translation
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MAPK signaling cascades
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Messenger RNA
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Elongation factors
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