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Research Article

Deconvoluting Post-Transplant Immunity: Cell Subset-Specific Mapping Reveals Pathways for Activation and Expansion of Memory T, Monocytes and B Cells

  • Yevgeniy A. Grigoryev,

    Affiliation: Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California, United States of America

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  • Sunil M. Kurian,

    Affiliation: Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California, United States of America

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  • Zafi Avnur,

    Affiliation: Roche, Palo Alto, California, United States of America

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  • Dominic Borie,

    Affiliation: Roche, Palo Alto, California, United States of America

    Current address: Amgen, Thousand Oaks, California, United States of America

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  • Jun Deng,

    Affiliation: Biomarker Discovery Sciences, Pharmaceutical Product Development (PPD), Menlo Park, California, United States of America

    Current address: Immune Tolerance Institute, Menlo Park, California, United States of America

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  • Daniel Campbell,

    Affiliations: Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California, United States of America, The Scripps Research Institute (TSRI) DNA Microarray Core Facility, La Jolla, California, United States of America

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  • Joanna Sung,

    Affiliation: Scripps Center for Organ and Cell Transplantation, La Jolla, California, United States of America

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  • Tania Nikolcheva,

    Affiliation: Roche, Palo Alto, California, United States of America

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  • Anthony Quinn,

    Affiliation: Roche, Palo Alto, California, United States of America

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  • Howard Schulman,

    Affiliation: Biomarker Discovery Sciences, Pharmaceutical Product Development (PPD), Menlo Park, California, United States of America

    Current address: Allosteros Therapeutics, Sunnyvale, California, United States of America

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  • Stanford L. Peng,

    Affiliation: Roche, Palo Alto, California, United States of America

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  • Randolph Schaffer,

    Affiliation: Scripps Center for Organ and Cell Transplantation, La Jolla, California, United States of America

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  • Jonathan Fisher,

    Affiliation: Scripps Center for Organ and Cell Transplantation, La Jolla, California, United States of America

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  • Tony Mondala,

    Affiliation: The Scripps Research Institute (TSRI) DNA Microarray Core Facility, La Jolla, California, United States of America

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  • Steven Head,

    Affiliation: The Scripps Research Institute (TSRI) DNA Microarray Core Facility, La Jolla, California, United States of America

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  • Stuart M. Flechner,

    Affiliation: Glickman Urological Institute, The Cleveland Clinic, Cleveland, Ohio, United States of America

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  • Aaron B. Kantor,

    Affiliation: Biomarker Discovery Sciences, Pharmaceutical Product Development (PPD), Menlo Park, California, United States of America

    Current address: Immune Tolerance Institute, Menlo Park, California, United States of America

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  • Christopher Marsh,

    Affiliations: Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California, United States of America, Scripps Center for Organ and Cell Transplantation, La Jolla, California, United States of America

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  • Daniel R. Salomon mail

    dsalomon@scripps.edu

    Affiliations: Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California, United States of America, Scripps Center for Organ and Cell Transplantation, La Jolla, California, United States of America

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  • Published: October 14, 2010
  • DOI: 10.1371/journal.pone.0013358

About the Authors

Yevgeniy A. Grigoryev, Sunil M. Kurian, Daniel Campbell, Christopher Marsh, Daniel R. Salomon
Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California, United States of America
Jun Deng, Howard Schulman, Aaron B. Kantor
Biomarker Discovery Sciences, Pharmaceutical Product Development (PPD), Menlo Park, California, United States of America
Joanna Sung, Randolph Schaffer, Jonathan Fisher, Christopher Marsh, Daniel R. Salomon
Scripps Center for Organ and Cell Transplantation, La Jolla, California, United States of America
Zafi Avnur, Dominic Borie, Tania Nikolcheva, Anthony Quinn, Stanford L. Peng
Roche, Palo Alto, California, United States of America
Daniel Campbell, Tony Mondala, Steven Head
The Scripps Research Institute (TSRI) DNA Microarray Core Facility, La Jolla, California, United States of America
Stuart M. Flechner
Glickman Urological Institute, The Cleveland Clinic, Cleveland, Ohio, United States of America

Corresponding Author

Email: dsalomon@scripps.edu

Competing Interests

There are two competing interests involved in this study. 1) Roche Pharmaceuticals employed the following authors: Z. Avnur, D. Borie, T. Nikolcheva, A. Quinn, and S.L. Peng. Roche funded part of this work as an unrestricted research grant to Dr. Daniel R. Salomon, the senior author. The rest of the work was supported by NIH grant AI063603 to Dr. Salomon. These employees of Roche were scientific collaborators and involved in the development of the study design and reviewed the first part of the data generated. Roche has relinquished any patent interests or IP ownership in anything involved in this project. Dr. Salomon and his collaborating scientists at Scripps and The Cleveland Clinic have no ongoing consultant or speaker arrangements of any kind with Roche and no stock, stock options or other forms of equity in Roche. 2) PPD employed the following authors during the time this work was done: J. Deng, A.B. Kantor, and H. Schulman. PPD provided the Surroscan high throughput flow cytometry work in the manuscript and their participation was funded by Roche as a combination of fee for service, and then consultant fees for the data evaluation and presentation phase of the Roche-funded portion of the work. PPD has never had any patent, IP or other rights in this work. These competing interests do not alter the authors' adherence to all the PLoS ONE policies on sharing data and materials as outlined at the journal website online.

Author Contributions

Conceived and designed the experiments: SMK DRS. Performed the experiments: YAG SMK DC. Analyzed the data: YAG SMK JD ABK. Contributed reagents/materials/analysis tools: ZA DB JS TN AQ HS SLP RLS JSF TM SRH SMF ABK CLM. Wrote the paper: YAG DRS.