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Research Article

A Multi-Parameter, High-Content, High-Throughput Screening Platform to Identify Natural Compounds that Modulate Insulin and Pdx1 Expression

  • Jessica A. Hill equal contributor,

    equal contributor Contributed equally to this work with: Jessica A. Hill, Marta Szabat, Corinne A. Hoesli

    Affiliation: Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, British Columbia, Canada

    X
  • Marta Szabat equal contributor,

    equal contributor Contributed equally to this work with: Jessica A. Hill, Marta Szabat, Corinne A. Hoesli

    Affiliation: Department of Chemical and Biological Engineering, University of British Columbia, Vancouver, British Columbia, Canada

    X
  • Corinne A. Hoesli equal contributor,

    equal contributor Contributed equally to this work with: Jessica A. Hill, Marta Szabat, Corinne A. Hoesli

    Affiliation: Department of Chemical and Biological Engineering, University of British Columbia, Vancouver, British Columbia, Canada

    X
  • Blair K. Gage,

    Affiliation: Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, British Columbia, Canada

    X
  • Yu Hsuan C. Yang,

    Affiliation: Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, British Columbia, Canada

    X
  • David E. Williams,

    Affiliation: Departments of Chemistry and Earth and Ocean Sciences, University of British Columbia, Vancouver, British Columbia, Canada

    X
  • Michael J. Riedel,

    Affiliation: Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, British Columbia, Canada

    X
  • Dan S. Luciani,

    Affiliation: Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, British Columbia, Canada

    X
  • Tatyana B. Kalynyak,

    Affiliation: Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, British Columbia, Canada

    X
  • Kevin Tsai,

    Affiliation: Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, British Columbia, Canada

    X
  • Ziliang Ao,

    Affiliation: Department of Surgery, University of British Columbia, Vancouver, British Columbia, Canada

    X
  • Raymond J. Andersen,

    Affiliation: Departments of Chemistry and Earth and Ocean Sciences, University of British Columbia, Vancouver, British Columbia, Canada

    X
  • Garth L. Warnock,

    Affiliation: Department of Surgery, University of British Columbia, Vancouver, British Columbia, Canada

    X
  • James M. Piret,

    Affiliation: Department of Chemical and Biological Engineering, University of British Columbia, Vancouver, British Columbia, Canada

    X
  • Timothy J. Kieffer,

    Affiliations: Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, British Columbia, Canada, Department of Surgery, University of British Columbia, Vancouver, British Columbia, Canada

    X
  • James D. Johnson mail

    jimjohn@interchange.ubc.ca

    Affiliations: Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, British Columbia, Canada, Department of Surgery, University of British Columbia, Vancouver, British Columbia, Canada

    X
  • Published: September 23, 2010
  • DOI: 10.1371/journal.pone.0012958

About the Authors

Jessica A. Hill, Blair K. Gage, Yu Hsuan C. Yang, Michael J. Riedel, Dan S. Luciani, Tatyana B. Kalynyak, Kevin Tsai, Timothy J. Kieffer, James D. Johnson
Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, British Columbia, Canada
Ziliang Ao, Garth L. Warnock, Timothy J. Kieffer, James D. Johnson
Department of Surgery, University of British Columbia, Vancouver, British Columbia, Canada
Marta Szabat, Corinne A. Hoesli, James M. Piret
Department of Chemical and Biological Engineering, University of British Columbia, Vancouver, British Columbia, Canada
David E. Williams, Raymond J. Andersen
Departments of Chemistry and Earth and Ocean Sciences, University of British Columbia, Vancouver, British Columbia, Canada

Corresponding Author

Email: jimjohn@interchange.ubc.ca

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: JAH MS CAH BKG YHCY DEW MJR DSL TBK RJA JMP TJK JDJ. Performed the experiments: JAH MS CAH BKG YHCY DEW MJR DSL TBK KT JDJ. Analyzed the data: JAH MS CAH YHCY DEW TBK KT JDJ. Contributed reagents/materials/analysis tools: JAH MS CAH DEW ZA RJA GW JMP TJK JDJ. Wrote the paper: JAH MS CAH DEW JDJ.