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Research Article

High-Throughput SuperSAGE for Digital Gene Expression Analysis of Multiple Samples Using Next Generation Sequencing

  • Hideo Matsumura mail,

    hideoma@shinshu-u.ac.jp

    Affiliations: Iwate Biotechnology Research Center, Kitakami, Japan, Gene Research Center, Shinshu University, Ueda, Japan

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  • Kentaro Yoshida,

    Affiliation: Iwate Biotechnology Research Center, Kitakami, Japan

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  • Shujun Luo,

    Affiliation: Illumina, Inc., Hayward, California, United States of America

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  • Eiji Kimura,

    Affiliation: Department of Anatomy, Iwate Medical University, Morioka, Japan

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  • Takahiro Fujibe,

    Affiliation: Iwate Biotechnology Research Center, Kitakami, Japan

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  • Zayed Albertyn,

    Affiliation: Centre for Comparative Genomics, Murdoch University, Perth, Australia

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  • Roberto A. Barrero,

    Affiliation: Centre for Comparative Genomics, Murdoch University, Perth, Australia

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  • Detlev H. Krüger,

    Affiliation: Institute of Medical Virology, University Hospital Charite, Berlin, Germany

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  • Günter Kahl,

    Affiliation: Molecular BioSciences, Biocentre, University of Frankfurt am Main, Frankfurt, Germany

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  • Gary P. Schroth,

    Affiliation: Illumina, Inc., Hayward, California, United States of America

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  • Ryohei Terauchi

    Affiliation: Iwate Biotechnology Research Center, Kitakami, Japan

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  • Published: August 06, 2010
  • DOI: 10.1371/journal.pone.0012010

About the Authors

Hideo Matsumura, Kentaro Yoshida, Takahiro Fujibe, Ryohei Terauchi
Iwate Biotechnology Research Center, Kitakami, Japan
Hideo Matsumura
Gene Research Center, Shinshu University, Ueda, Japan
Shujun Luo, Gary P. Schroth
Illumina, Inc., Hayward, California, United States of America
Eiji Kimura
Department of Anatomy, Iwate Medical University, Morioka, Japan
Zayed Albertyn, Roberto A. Barrero
Centre for Comparative Genomics, Murdoch University, Perth, Australia
Detlev H. Krüger
Institute of Medical Virology, University Hospital Charite, Berlin, Germany
Günter Kahl
Molecular BioSciences, Biocentre, University of Frankfurt am Main, Frankfurt, Germany

Corresponding Author

Email: hideoma@shinshu-u.ac.jp

Competing Interests

Shujun Luo and Gary P. Schroth are employees of Illumina Inc. This does not alter the authors' adherence to all the PLoS ONE policies on sharing data and materials.

Author Contributions

Conceived and designed the experiments: HM DHK GK GPS RT. Performed the experiments: HM KY SL EK TF. Analyzed the data: HM KY ZA RAB RT. Contributed reagents/materials/analysis tools: SL EK TF. Wrote the paper: HM DHK GK GPS RT.