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Research Article

DMSO and Betaine Greatly Improve Amplification of GC-Rich Constructs in De Novo Synthesis

  • Michael A. Jensen mail,

    m.a.jensen@stanford.edu

    Affiliation: Stanford Genome Technology Center, Stanford University, Palo Alto, California, United States of America

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  • Marilyn Fukushima,

    Affiliation: Stanford Genome Technology Center, Stanford University, Palo Alto, California, United States of America

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  • Ronald W. Davis

    Affiliation: Stanford Genome Technology Center, Stanford University, Palo Alto, California, United States of America

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  • Published: June 11, 2010
  • DOI: 10.1371/journal.pone.0011024
  • Featured in PLOS Collections

About the Authors

Michael A. Jensen, Marilyn Fukushima, Ronald W. Davis
Stanford Genome Technology Center, Stanford University, Palo Alto, California, United States of America

Corresponding Author

Email: m.a.jensen@stanford.edu

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: MJ. Performed the experiments: MF. Analyzed the data: MJ. Contributed reagents/materials/analysis tools: MJ RWD. Wrote the paper: MJ. Principal investigator: RWD.