About the Authors

Siu-Hong Chan

Affiliation Research Department, New England Biolabs, Inc., Ipswich, Massachusetts, United States of America

Lars Opitz

Current address: Value Stream Secondary, GlaxoSmithKline Biologicals, Dresden, Germany

Affiliation Research Department, New England Biolabs, Inc., Ipswich, Massachusetts, United States of America

Lauren Higgins

Affiliation Research Department, New England Biolabs, Inc., Ipswich, Massachusetts, United States of America

Diana O'loane

Affiliation Research Department, New England Biolabs, Inc., Ipswich, Massachusetts, United States of America

Shuang-yong Xu

xus@neb.com

Affiliation Research Department, New England Biolabs, Inc., Ipswich, Massachusetts, United States of America

Competing Interests

SHC SYX DO LH are employees of New England Biolabs (NEB), Inc. This employment does not alter the author's adherence to all the PLoS ONE policies on sharing data and materials. NEB is a commercial source of HpyAV and KpnI restriction endonucleases. LO was a one-year visiting student working on a Master degree thesis (supported by NEB).

Author Contributions

Conceived and designed the experiments: SHC SyX. Performed the experiments: SHC LO LH SyX. Analyzed the data: SHC LO LH SyX. Contributed reagents/materials/analysis tools: SHC DO SyX. Wrote the paper: SHC SyX.