About the Authors

W. Ray Waters

ray.waters@ars.usda.gov

Affiliation National Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Ames, Iowa, United States of America

Mitchell V. Palmer

Affiliation National Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Ames, Iowa, United States of America

Brian J. Nonnecke

Affiliation National Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Ames, Iowa, United States of America

Tyler C. Thacker

Affiliation National Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Ames, Iowa, United States of America

D. Mark Estes

Affiliation University of Texas Medical Branch, Department of Pediatrics and the Sealy Center for Vaccine Development, Galveston, Texas, United States of America

Michelle H. Larsen

Affiliation Howard Hughes Medical Institute and Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York, United States of America

William R. Jacobs Jr

Affiliation Howard Hughes Medical Institute and Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York, United States of America

Peter Andersen

Affiliation Statens Serum Institute, Copenhagen, Denmark

James McNair

Affiliation Bacteriology Department, Veterinary Sciences Division, Stormont, Belfast

F. C. Minion

Affiliation Veterinary Microbiology and Preventive Medicine, Iowa State University, Ames, Iowa, United States of America

Konstantin P. Lyashchenko

Affiliation Chembio Diagnostic Systems, Medford, New York, United States of America

R. Glyn Hewinson

Affiliation UK Veterinary Laboratories Agency, Weybridge, New Haw, Addlestone, Surrey, United Kingdom

H. Martin Vordermeier

Affiliation UK Veterinary Laboratories Agency, Weybridge, New Haw, Addlestone, Surrey, United Kingdom

Randy E. Sacco

Affiliation National Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Ames, Iowa, United States of America

Competing Interests

Peter Andersen is co-inventor of patents relating to TB antigens, ESAT-6 and CFP-10. All rights have been assigned to Statens Serum Institut.

Author Contributions

Conceived and designed the experiments: WRW MVP BJN TCT DME RES. Performed the experiments: WRW MVP TCT MHL WRJ RES. Analyzed the data: WRW MVP BJN RES. Contributed reagents/materials/analysis tools: WRW MVP BJN DME MHL WRJ PA JM CM KL GH MV RES. Wrote the paper: WRW DME RES.