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Research Article

LIF-Free Embryonic Stem Cell Culture in Simulated Microgravity

  • Yumi Kawahara,

    Affiliations: Department of Clinical Neuroscience and Therapeutics, Graduate School of Biomedical Sciences, Hiroshima University, Minami-ku, Hiroshima, Japan, Space-Bio Laboratories Y. K., Hiroshima University, Minami-ku, Hiroshima, Japan

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  • Tomotaka Manabe,

    Affiliation: Division of Bio-Environmental Adaptation Sciences, Graduate School of Health Sciences, Hiroshima University, Minami-ku, Hiroshima, Japan

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  • Masaya Matsumoto,

    Affiliation: Division of Bio-Environmental Adaptation Sciences, Graduate School of Health Sciences, Hiroshima University, Minami-ku, Hiroshima, Japan

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  • Teruyuki Kajiume,

    Affiliation: Department of Pediatrics, Graduate School of Biomedical Sciences, Hiroshima University, Minami-ku, Hiroshima, Japan

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  • Masayasu Matsumoto,

    Affiliation: Department of Clinical Neuroscience and Therapeutics, Graduate School of Biomedical Sciences, Hiroshima University, Minami-ku, Hiroshima, Japan

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  • Louis Yuge mail

    ryuge@hiroshima-u.ac.jp

    Affiliations: Space-Bio Laboratories Y. K., Hiroshima University, Minami-ku, Hiroshima, Japan, Division of Bio-Environmental Adaptation Sciences, Graduate School of Health Sciences, Hiroshima University, Minami-ku, Hiroshima, Japan

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  • Published: July 23, 2009
  • DOI: 10.1371/journal.pone.0006343

About the Authors

Yumi Kawahara, Masayasu Matsumoto
Department of Clinical Neuroscience and Therapeutics, Graduate School of Biomedical Sciences, Hiroshima University, Minami-ku, Hiroshima, Japan
Yumi Kawahara, Louis Yuge
Space-Bio Laboratories Y. K., Hiroshima University, Minami-ku, Hiroshima, Japan
Tomotaka Manabe, Masaya Matsumoto, Louis Yuge
Division of Bio-Environmental Adaptation Sciences, Graduate School of Health Sciences, Hiroshima University, Minami-ku, Hiroshima, Japan
Teruyuki Kajiume
Department of Pediatrics, Graduate School of Biomedical Sciences, Hiroshima University, Minami-ku, Hiroshima, Japan

Corresponding Author

Email: ryuge@hiroshima-u.ac.jp

Competing Interests

Louis Yuge and MITSUBISHI HEAVY INDUSTRIES, LTD.(MHI) have a patent application on the 3D-clinostat, Japanese patent (publication number P2001-197182A, date of filing June 28, 2001, and overseas patents, WO2004/061092 A1 PCT, 2004). This has no influence on the content of this article. Our laboratory has a research agreement with MHI.

Author Contributions

Conceived and designed the experiments: YK TK LY. Performed the experiments: YK TM Masaya Matsumoto TK. Analyzed the data: YK Masayasu Matsumoto LY. Contributed reagents/materials/analysis tools: LY. Wrote the paper: YK LY.