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Research Article

Concurrent Growth Rate and Transcript Analyses Reveal Essential Gene Stringency in Escherichia coli

  • Shan Goh,

    Affiliations: Department of Cell and Molecular Biology, Karolinska Institute, Stockholm, Sweden, Department of Pathology and Infectious Diseases, Royal Veterinary College, University of London, London, United Kingdom

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  • Jaroslaw M. Boberek,

    Affiliation: Department of Pathology and Infectious Diseases, Royal Veterinary College, University of London, London, United Kingdom

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  • Nobutaka Nakashima,

    Affiliation: Research Institute of Genome-based biofactory, Toyohira-Ku, Sapporo, Japan

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  • Jem Stach,

    Affiliation: School of Biology, University of Newcastle, Newcastle upon Tyne, United Kingdom

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  • Liam Good mail

    lgood@rvc.ac.uk

    Affiliations: Department of Cell and Molecular Biology, Karolinska Institute, Stockholm, Sweden, Department of Pathology and Infectious Diseases, Royal Veterinary College, University of London, London, United Kingdom

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  • Published: June 26, 2009
  • DOI: 10.1371/journal.pone.0006061

About the Authors

Shan Goh, Liam Good
Department of Cell and Molecular Biology, Karolinska Institute, Stockholm, Sweden
Shan Goh, Jaroslaw M. Boberek, Liam Good
Department of Pathology and Infectious Diseases, Royal Veterinary College, University of London, London, United Kingdom
Nobutaka Nakashima
Research Institute of Genome-based biofactory, Toyohira-Ku, Sapporo, Japan
Jem Stach
School of Biology, University of Newcastle, Newcastle upon Tyne, United Kingdom

Corresponding Author

Email: lgood@rvc.ac.uk

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: LG. Performed the experiments: SG. Analyzed the data: SG LG. Wrote the paper: SG LG. Carried out cloning of fabI, ftsZ and murA over-expressing strains and all antisense RNA rescue experiments: JMB. Carried out cloning of acpP over-expressing strain: NN. Helped design and supervise rescue experiments: JS.