In Vitro Recombination Catalyzed by Bacterial Class 1 Integron Integrase IntI1 Involves Cooperative Binding and Specific Oligomeric Intermediates

Véronique Dubois; Carole Debreyer; Claudine Quentin; Vincent Parissi

doi:10.1371/journal.pone.0005228

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Peer-reviewed

Research Article

In Vitro Recombination Catalyzed by Bacterial Class 1 Integron Integrase IntI1 Involves Cooperative Binding and Specific Oligomeric Intermediates

Véronique Dubois,

Affiliation Laboratory of Cellular and Molecular Microbiology and Pathogenicity (MCMP), UMR 5097-CNRS, University Victor Segalen Bordeaux 2, Bordeaux, France
⨯
Carole Debreyer,

Affiliation Laboratory of Cellular and Molecular Microbiology and Pathogenicity (MCMP), UMR 5097-CNRS, University Victor Segalen Bordeaux 2, Bordeaux, France
⨯
Claudine Quentin,

Affiliation Laboratory of Cellular and Molecular Microbiology and Pathogenicity (MCMP), UMR 5097-CNRS, University Victor Segalen Bordeaux 2, Bordeaux, France
⨯
Vincent Parissi

* E-mail: vincent.parissi@reger.u-bordeaux2.fr

Affiliation Laboratory of Cellular and Molecular Microbiology and Pathogenicity (MCMP), UMR 5097-CNRS, University Victor Segalen Bordeaux 2, Bordeaux, France
⨯

In Vitro Recombination Catalyzed by Bacterial Class 1 Integron Integrase IntI1 Involves Cooperative Binding and Specific Oligomeric Intermediates

Véronique Dubois,
Carole Debreyer,
Claudine Quentin,
Vincent Parissi

Published: April 21, 2009
https://doi.org/10.1371/journal.pone.0005228

About the Authors

Véronique Dubois

Affiliation Laboratory of Cellular and Molecular Microbiology and Pathogenicity (MCMP), UMR 5097-CNRS, University Victor Segalen Bordeaux 2, Bordeaux, France

Carole Debreyer

Affiliation Laboratory of Cellular and Molecular Microbiology and Pathogenicity (MCMP), UMR 5097-CNRS, University Victor Segalen Bordeaux 2, Bordeaux, France

Claudine Quentin

Affiliation Laboratory of Cellular and Molecular Microbiology and Pathogenicity (MCMP), UMR 5097-CNRS, University Victor Segalen Bordeaux 2, Bordeaux, France

Vincent Parissi

* E-mail: vincent.parissi@reger.u-bordeaux2.fr

Affiliation Laboratory of Cellular and Molecular Microbiology and Pathogenicity (MCMP), UMR 5097-CNRS, University Victor Segalen Bordeaux 2, Bordeaux, France

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: VP. Performed the experiments: VD CD VP. Analyzed the data: CD CQ VP. Contributed reagents/materials/analysis tools: VD VP. Wrote the paper: VP.

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Recombination reactions
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Recombinant proteins
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Recombination-based assay
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Radiolabeling
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Antibiotic resistance
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Dimers
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Gel electrophoresis
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Nucleases
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