About the Authors
- Jessica L. Dermody
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Affiliation Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts, United States of America
- Jonathan M. Dreyfuss
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Affiliation Harvard-Partners Center for Genetics and Genomics, Boston, Massachusetts, United States of America
- Judit Villén
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Affiliation Department of Cell Biology, Harvard Medical School, Boston, Massachusetts, United States of America
- Babatunde Ogundipe
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Affiliation Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts, United States of America
- Steven P. Gygi
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Affiliation Department of Cell Biology, Harvard Medical School, Boston, Massachusetts, United States of America
- Peter J. Park
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Affiliation Harvard-Partners Center for Genetics and Genomics, Boston, Massachusetts, United States of America
- Alfred S. Ponticelli
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Affiliation Department of Biochemistry, School of Medicine and Biomedical Sciences, State University of New York, Buffalo, New York, United States of America
- Claire L. Moore
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Affiliation Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts, United States of America
- Stephen Buratowski
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Affiliation Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts, United States of America
- Miriam E. Bucheli
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* E-mail: Miriam_Bucheli@hms.harvard.edu
Affiliation Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts, United States of America
Competing Interests
The authors have declared that no competing interests exist.
Author Contributions
Conceived and designed the experiments: MEB. Performed the experiments: JLD JV BO MEB. Analyzed the data: JLD JD JV MEB. Contributed reagents/materials/analysis tools: JD JV SPG PJP ASP CM SB MEB. Wrote the paper: JLD MEB. Figures 2 & 4: JLD. Oligo(dC)-tail template elongation assay: JLD. Figure 5, Expression analysis: JD PJP. Figure S2, S3 and S4, Expression analysis: JD PJP. Figure S1: JV SPG. Mass spectrometry analysis of Cka1 phosphorylation specificity using peptide libraries: JV. Analysis of Npl3 phosphorylation sites: JV. Editing of paper: JV. Figure 3: BO. Cka1 phosphorylation in vitro analysis: BO. Cka1 phosphorylation in vivo analysis: BO. Mass spectrometry analysis of Cka1 phosphorylation specificity using peptide libraries: SPG. Analysis of Npl3 phosphorylation sites: SPG. Figure 2: ASP. RNAP II purification: ASP. Editing of paper and guidance: CM SB.