Study of a Synthetic Human Olfactory Receptor 17-4: Expression and Purification from an Inducible Mammalian Cell Line

Brian L. Cook; Karin E. Ernberg; Hyeyoun Chung; Shuguang Zhang

doi:10.1371/journal.pone.0002920

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Open Access

Peer-reviewed

Research Article

Study of a Synthetic Human Olfactory Receptor 17-4: Expression and Purification from an Inducible Mammalian Cell Line

Brian L. Cook,

Affiliation Department of Biological Engineering and Center for Biomedical Engineering NE47-379, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America
⨯
Karin E. Ernberg,

Affiliation Department of Biological Engineering and Center for Biomedical Engineering NE47-379, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America
⨯
Hyeyoun Chung,

Affiliation Department of Biological Engineering and Center for Biomedical Engineering NE47-379, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America
⨯
Shuguang Zhang

* E-mail: shuguang@mit.edu

Affiliation Department of Biological Engineering and Center for Biomedical Engineering NE47-379, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America
⨯

Study of a Synthetic Human Olfactory Receptor 17-4: Expression and Purification from an Inducible Mammalian Cell Line

Brian L. Cook,
Karin E. Ernberg,
Hyeyoun Chung,
Shuguang Zhang

Published: August 6, 2008
https://doi.org/10.1371/journal.pone.0002920

About the Authors

Brian L. Cook

Affiliation Department of Biological Engineering and Center for Biomedical Engineering NE47-379, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America

Karin E. Ernberg

Affiliation Department of Biological Engineering and Center for Biomedical Engineering NE47-379, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America

Hyeyoun Chung

Affiliation Department of Biological Engineering and Center for Biomedical Engineering NE47-379, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America

Shuguang Zhang

* E-mail: shuguang@mit.edu

Affiliation Department of Biological Engineering and Center for Biomedical Engineering NE47-379, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: BLC SZ. Performed the experiments: BLC KEE HC. Analyzed the data: BLC KEE HC SZ. Contributed reagents/materials/analysis tools: BLC. Wrote the paper: BLC SZ.

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Olfactory receptors
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Tetracyclines
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Monomers
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Membrane proteins
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Elution
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G protein coupled receptors
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Olfactory receptor neurons
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Polymerase chain reaction
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