Heterologous Protein Expression Is Enhanced by Harmonizing the Codon Usage Frequencies of the Target Gene with those of the Expression Host

Evelina Angov; Collette J. Hillier; Randall L. Kincaid; Jeffrey A. Lyon

doi:10.1371/journal.pone.0002189

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Peer-reviewed

Research Article

Heterologous Protein Expression Is Enhanced by Harmonizing the Codon Usage Frequencies of the Target Gene with those of the Expression Host

Evelina Angov ,

* E-mail: Evelina.angov@us.army.mil

Affiliation Molecular Parasitology, Division of Malaria Vaccine Development, Walter Reed Army Institute of Research, Silver Spring, Maryland, United States of America
⨯
Collette J. Hillier,

Affiliation Molecular Parasitology, Division of Malaria Vaccine Development, Walter Reed Army Institute of Research, Silver Spring, Maryland, United States of America
⨯
Randall L. Kincaid,

Affiliation Veritas, Inc., Rockville, Maryland, United States of America
⨯
Jeffrey A. Lyon

Affiliation Molecular Parasitology, Division of Malaria Vaccine Development, Walter Reed Army Institute of Research, Silver Spring, Maryland, United States of America
⨯

Heterologous Protein Expression Is Enhanced by Harmonizing the Codon Usage Frequencies of the Target Gene with those of the Expression Host

Evelina Angov,
Collette J. Hillier,
Randall L. Kincaid,
Jeffrey A. Lyon

Published: May 14, 2008
https://doi.org/10.1371/journal.pone.0002189

About the Authors

Evelina Angov

* E-mail: Evelina.angov@us.army.mil

Affiliation Molecular Parasitology, Division of Malaria Vaccine Development, Walter Reed Army Institute of Research, Silver Spring, Maryland, United States of America

Collette J. Hillier

Affiliation Molecular Parasitology, Division of Malaria Vaccine Development, Walter Reed Army Institute of Research, Silver Spring, Maryland, United States of America

Randall L. Kincaid

Affiliation Veritas, Inc., Rockville, Maryland, United States of America

Jeffrey A. Lyon

Affiliation Molecular Parasitology, Division of Malaria Vaccine Development, Walter Reed Army Institute of Research, Silver Spring, Maryland, United States of America

Competing Interests

Coauthors, EA, RLK and JAL are listed as co-inventors on a patent application for the algorithm, codon harmonization.

Author Contributions

Conceived and designed the experiments: EA JL. Performed the experiments: EA JL CH. Analyzed the data: EA JL CH. Contributed reagents/materials/analysis tools: EA JL RK. Wrote the paper: EA JL RK.

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Protein structure
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Protein expression
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Plasmodium
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Plasmid construction
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Coomassie Blue staining
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Protein translation
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Algorithms
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Messenger RNA
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