Enriched Monolayer Precursor Cell Cultures from Micro-Dissected Adult Mouse Dentate Gyrus Yield Functional Granule Cell-Like Neurons

Harish Babu; Giselle Cheung; Helmut Kettenmann; Theo D. Palmer; Gerd Kempermann

doi:10.1371/journal.pone.0000388

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Peer-reviewed

Research Article

Enriched Monolayer Precursor Cell Cultures from Micro-Dissected Adult Mouse Dentate Gyrus Yield Functional Granule Cell-Like Neurons

Harish Babu,

Affiliations Max Delbrück Center for Molecular Medicine (MDC) Berlin-Buch, Berlin, Germany, VolkswagenStiftung Research Group, Department of Experimental Neurology, Charité University Medicine Berlin, Berlin, Germany
⨯
Giselle Cheung,

Affiliation Max Delbrück Center for Molecular Medicine (MDC) Berlin-Buch, Berlin, Germany
⨯
Helmut Kettenmann,

Affiliation Max Delbrück Center for Molecular Medicine (MDC) Berlin-Buch, Berlin, Germany
⨯
Theo D. Palmer,

Affiliation Department of Neurosurgery, Stanford University, Palo Alto, United States of America
⨯
Gerd Kempermann

*To whom correspondence should be addressed. E-mail: gerd.kempermann@mdc-berlin.de

Affiliations Max Delbrück Center for Molecular Medicine (MDC) Berlin-Buch, Berlin, Germany, VolkswagenStiftung Research Group, Department of Experimental Neurology, Charité University Medicine Berlin, Berlin, Germany
⨯

Enriched Monolayer Precursor Cell Cultures from Micro-Dissected Adult Mouse Dentate Gyrus Yield Functional Granule Cell-Like Neurons

Harish Babu,
Giselle Cheung,
Helmut Kettenmann,
Theo D. Palmer,
Gerd Kempermann

Published: April 25, 2007
https://doi.org/10.1371/journal.pone.0000388

About the Authors

Harish Babu

Affiliations Max Delbrück Center for Molecular Medicine (MDC) Berlin-Buch, Berlin, Germany, VolkswagenStiftung Research Group, Department of Experimental Neurology, Charité University Medicine Berlin, Berlin, Germany

Giselle Cheung

Affiliation Max Delbrück Center for Molecular Medicine (MDC) Berlin-Buch, Berlin, Germany

Helmut Kettenmann

Affiliation Max Delbrück Center for Molecular Medicine (MDC) Berlin-Buch, Berlin, Germany

Theo D. Palmer

Affiliation Department of Neurosurgery, Stanford University, Palo Alto, United States of America

Gerd Kempermann

*To whom correspondence should be addressed. E-mail: gerd.kempermann@mdc-berlin.de

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: GK HB. Performed the experiments: HB GC. Analyzed the data: HK TP HB GC. Contributed reagents/materials/analysis tools: HK TP. Wrote the paper: HB.

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Precursor cells
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Neuronal differentiation
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Dentate gyrus
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Hippocampus
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Neurons
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Granule cells
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Nestin
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Cell differentiation
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