B-RAF and N-RAS Mutations Are Preserved during Short Time In Vitro Propagation and Differentially Impact Prognosis

Selma Ugurel; Ranjit K. Thirumaran; Sandra Bloethner; Andreas Gast; Antje Sucker; Jan Mueller-Berghaus; Werner Rittgen; Kari Hemminki; Jürgen C. Becker; Rajiv Kumar; Dirk Schadendorf

doi:10.1371/journal.pone.0000236

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Open Access

Peer-reviewed

Research Article

B-RAF and N-RAS Mutations Are Preserved during Short Time In Vitro Propagation and Differentially Impact Prognosis

Selma Ugurel ,

*To whom correspondence should be addressed. E-mail: s.ugurel@dkfz.de

Affiliation Skin Cancer Unit, German Cancer Research Center Heidelberg and Department of Dermatology, University Medical Center Mannheim, Mannheim, Germany
⨯
Ranjit K. Thirumaran,

Affiliation Division of Molecular Genetic Epidemiology, German Cancer Research Center, Heidelberg, Germany
⨯
Sandra Bloethner,

Affiliation Division of Molecular Genetic Epidemiology, German Cancer Research Center, Heidelberg, Germany
⨯
Andreas Gast,

Affiliation Division of Molecular Genetic Epidemiology, German Cancer Research Center, Heidelberg, Germany
⨯
Antje Sucker,

Affiliation Skin Cancer Unit, German Cancer Research Center Heidelberg and Department of Dermatology, University Medical Center Mannheim, Mannheim, Germany
⨯
Jan Mueller-Berghaus,

Affiliation Skin Cancer Unit, German Cancer Research Center Heidelberg and Department of Dermatology, University Medical Center Mannheim, Mannheim, Germany
⨯
Werner Rittgen,

Affiliation Central Unit of Biostatistics, German Cancer Research Center, Heidelberg, Germany
⨯
Kari Hemminki,

Affiliations Division of Molecular Genetic Epidemiology, German Cancer Research Center, Heidelberg, Germany, Department of Biosciences, Karolinska Institute, Huddinge, Sweden
⨯
Jürgen C. Becker,

Affiliation Department of Dermatology, University of Wuerzburg, Germany
⨯
Rajiv Kumar,

Affiliations Division of Molecular Genetic Epidemiology, German Cancer Research Center, Heidelberg, Germany, Department of Biosciences, Karolinska Institute, Huddinge, Sweden
⨯
Dirk Schadendorf

Affiliation Skin Cancer Unit, German Cancer Research Center Heidelberg and Department of Dermatology, University Medical Center Mannheim, Mannheim, Germany
⨯

B-RAF and N-RAS Mutations Are Preserved during Short Time In Vitro Propagation and Differentially Impact Prognosis

Selma Ugurel,
Ranjit K. Thirumaran,
Sandra Bloethner,
Andreas Gast,
Antje Sucker,
Jan Mueller-Berghaus,
Werner Rittgen,
Kari Hemminki,
Jürgen C. Becker,
Rajiv Kumar

Published: February 21, 2007
https://doi.org/10.1371/journal.pone.0000236

About the Authors

Selma Ugurel

*To whom correspondence should be addressed. E-mail: s.ugurel@dkfz.de

Affiliation Skin Cancer Unit, German Cancer Research Center Heidelberg and Department of Dermatology, University Medical Center Mannheim, Mannheim, Germany

Ranjit K. Thirumaran

Affiliation Division of Molecular Genetic Epidemiology, German Cancer Research Center, Heidelberg, Germany

Sandra Bloethner

Affiliation Division of Molecular Genetic Epidemiology, German Cancer Research Center, Heidelberg, Germany

Andreas Gast

Affiliation Division of Molecular Genetic Epidemiology, German Cancer Research Center, Heidelberg, Germany

Antje Sucker

Affiliation Skin Cancer Unit, German Cancer Research Center Heidelberg and Department of Dermatology, University Medical Center Mannheim, Mannheim, Germany

Jan Mueller-Berghaus

Affiliation Skin Cancer Unit, German Cancer Research Center Heidelberg and Department of Dermatology, University Medical Center Mannheim, Mannheim, Germany

Werner Rittgen

Affiliation Central Unit of Biostatistics, German Cancer Research Center, Heidelberg, Germany

Kari Hemminki

Affiliations Division of Molecular Genetic Epidemiology, German Cancer Research Center, Heidelberg, Germany, Department of Biosciences, Karolinska Institute, Huddinge, Sweden

Jürgen C. Becker

Affiliation Department of Dermatology, University of Wuerzburg, Germany

Rajiv Kumar

Affiliations Division of Molecular Genetic Epidemiology, German Cancer Research Center, Heidelberg, Germany, Department of Biosciences, Karolinska Institute, Huddinge, Sweden

Dirk Schadendorf

Affiliation Skin Cancer Unit, German Cancer Research Center Heidelberg and Department of Dermatology, University Medical Center Mannheim, Mannheim, Germany

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: SU RK DS. Performed the experiments: RT SB AG AS JM. Analyzed the data: WR JB SU RK. Contributed reagents/materials/analysis tools: KH SU RK DS. Wrote the paper: JB SU RK.

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