About the Authors

Branden S. Moriarity

Affiliations Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, Minnesota, United States of America, Center for Genome Engineering and Institute of Human Genetics, University of Minnesota, Minneapolis, Minnesota, United States of America, Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota, United States of America

Eric P. Rahrmann

Affiliations Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, Minnesota, United States of America, Center for Genome Engineering and Institute of Human Genetics, University of Minnesota, Minneapolis, Minnesota, United States of America, Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota, United States of America

Dominic A. Beckmann

Affiliations Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, Minnesota, United States of America, Center for Genome Engineering and Institute of Human Genetics, University of Minnesota, Minneapolis, Minnesota, United States of America, Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota, United States of America

Caitlin B. Conboy

Affiliations Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, Minnesota, United States of America, Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota, United States of America

Adrienne L. Watson

Affiliations Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, Minnesota, United States of America, Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota, United States of America

Daniel F. Carlson

Affiliations Center for Genome Engineering and Institute of Human Genetics, University of Minnesota, Minneapolis, Minnesota, United States of America, Department of Animal Science, University of Minnesota, Minneapolis, Minnesota, United States of America, Recombinetics, Inc., Saint Paul, Minnesota, United States of America

Erik R. Olson

Affiliation Discovery Genomics, Inc, Minneapolis, Minnesota, United States of America

Kendra A. Hyland

Affiliation Discovery Genomics, Inc, Minneapolis, Minnesota, United States of America

Scott C. Fahrenkrug

Affiliations Center for Genome Engineering and Institute of Human Genetics, University of Minnesota, Minneapolis, Minnesota, United States of America, Department of Animal Science, University of Minnesota, Minneapolis, Minnesota, United States of America, Recombinetics, Inc., Saint Paul, Minnesota, United States of America

R. Scott McIvor

Affiliations Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, Minnesota, United States of America, Center for Genome Engineering and Institute of Human Genetics, University of Minnesota, Minneapolis, Minnesota, United States of America, Discovery Genomics, Inc, Minneapolis, Minnesota, United States of America

David A. Largaespada

larga002@umn.edu

Affiliations Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, Minnesota, United States of America, Center for Genome Engineering and Institute of Human Genetics, University of Minnesota, Minneapolis, Minnesota, United States of America, Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota, United States of America, Discovery Genomics, Inc, Minneapolis, Minnesota, United States of America, Department of Pediatrics, University of Minnesota, Minneapolis, Minnesota, United States of America

Competing Interests

R.S.M is a founder and managing employee of Discovery Genomics, Inc. (DGI), including stock and options. K.H. and E.O. are employees of DGI with stock options. D.A.L. is a founder of DGI with stock. D.A.L. is a founder of NeoClone Biotechnology, Inc. with stock. D.F.C and S.F are currently an employee of and CEO, respectfully, of Recombinetics, Inc. There are no patents, products in development or marketed products to declare. This does not alter the authors' adherence to all the PLOS ONE policies on sharing data and materials.

Author Contributions

Conceived and designed the experiments: BSM DAL RSM. Performed the experiments: BSM DAB EPR CBC ALW KAH. Analyzed the data: BSM DAB EPR. Contributed reagents/materials/analysis tools: DFC SCF ERO. Wrote the paper: BSM.