About the Authors

Žaklina Strezoska

Affiliation Molecular Biology, Thermo Fisher Scientific, Lafayette, Colorado, United States of America

Abel Licon

Affiliation Molecular Biology, Thermo Fisher Scientific, Lafayette, Colorado, United States of America

Josh Haimes

Affiliation Molecular Biology, Thermo Fisher Scientific, Lafayette, Colorado, United States of America

Katie Jansen Spayd

Affiliation Molecular Biology, Thermo Fisher Scientific, Lafayette, Colorado, United States of America

Kruti M. Patel

Affiliation Molecular Biology, Thermo Fisher Scientific, Lafayette, Colorado, United States of America

Kevin Sullivan

Affiliation Molecular Biology, Thermo Fisher Scientific, Lafayette, Colorado, United States of America

Katarzyna Jastrzebski

Affiliation Victorian Centre for Functional Genomics, Peter MacCallum Cancer Centre, East Melbourne, Victoria, Australia

Kaylene J. Simpson

Affiliations Victorian Centre for Functional Genomics, Peter MacCallum Cancer Centre, East Melbourne, Victoria, Australia, The Sir Peter MacCallum Department of Oncology, The University of Melbourne, Parkville, Victoria, Australia

Devin Leake

Affiliation Molecular Biology, Thermo Fisher Scientific, Lafayette, Colorado, United States of America

Anja van Brabant Smith

Affiliation Molecular Biology, Thermo Fisher Scientific, Lafayette, Colorado, United States of America

Annaleen Vermeulen

annleen.vermeulen@thermofisher.com

Affiliation Molecular Biology, Thermo Fisher Scientific, Lafayette, Colorado, United States of America

Competing Interests

Z. Strezoska, A. Licon, J. Haimes, K.J. Spayd, K.M. Patel, K. Sullivan, D. Leake and A. Vermeulen are employed or were formerly employed by Thermo Fisher Scientific, one of the funders of this study. Some of the materials used in this study are products sold by Thermo Fisher Scientific (including Decode RNAi Annotated Genome Screening Library, Nanodrop 1000, Phusion® Hot Start II High-Fidelity DNA Polymerase, ABsolute™ Blue qPCR SYBR® Green master mix and Matrix PlateMate) and are subject to use restrictions or licenses. There are no further patents, products in development or marketed products referenced in this article to declare. This does not alter the authors' adherence to all the PLoS ONE policies on sharing data and materials, as detailed online in the guide for authors. K. Jastrzebski and K.J. Simpson declare that they have no competing interests.

Author Contributions

Conceived and designed the experiments: ŽS AL JH K. J. Spayd KMP K. J. Simpson KJ DL AvBS AV. Performed the experiments: ŽS JH K. J. Spayd KMP. Analyzed the data: ŽS JH K. J. Spayd KMP KS AL DL AvBS AV. Contributed reagents/materials/analysis tools: AL KS. Wrote the paper: ZS JH K. J. Spayd KMP AL DL AvBS AV.