Transient Gene Expression in Serum-Free Suspension-Growing Mammalian Cells for the Production of Foot-and-Mouth Disease Virus Empty Capsids

Ana Clara Mignaqui; Vanesa Ruiz; Sylvie Perret; Gilles St-Laurent; Parminder Singh Chahal; Julia Transfiguracion; Ayelén Sammarruco; Victoria Gnazzo; Yves Durocher; Andrés Wigdorovitz

doi:10.1371/journal.pone.0072800

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Open Access

Peer-reviewed

Research Article

Transient Gene Expression in Serum-Free Suspension-Growing Mammalian Cells for the Production of Foot-and-Mouth Disease Virus Empty Capsids

Ana Clara Mignaqui ,

* E-mail: amignaqui@cnia.inta.gov.ar

Affiliations Instituto de Virología, Centro de Investigación en Ciencias Veterinarias y Agronómicas, Instituto Nacional de Tecnología Agropecuaria (INTA), Hurlingham, Buenos Aires, Argentina, Consejo Nacional de Investigaciones Científicas y Tecnológicas, Ciudad Autónoma de Buenos Aires, Buenos Aires, Argentina
⨯
Vanesa Ruiz,

Affiliations Instituto de Virología, Centro de Investigación en Ciencias Veterinarias y Agronómicas, Instituto Nacional de Tecnología Agropecuaria (INTA), Hurlingham, Buenos Aires, Argentina, Consejo Nacional de Investigaciones Científicas y Tecnológicas, Ciudad Autónoma de Buenos Aires, Buenos Aires, Argentina
⨯
Sylvie Perret,

Affiliation Biotechnology Research Institute, National Research Council of Canada, Montreal, Quebec, Canada
⨯
Gilles St-Laurent,

Affiliation Biotechnology Research Institute, National Research Council of Canada, Montreal, Quebec, Canada
⨯
Parminder Singh Chahal,

Affiliation Biotechnology Research Institute, National Research Council of Canada, Montreal, Quebec, Canada
⨯
Julia Transfiguracion,

Affiliation Biotechnology Research Institute, National Research Council of Canada, Montreal, Quebec, Canada
⨯
Ayelén Sammarruco,

Affiliation Instituto de Virología, Centro de Investigación en Ciencias Veterinarias y Agronómicas, Instituto Nacional de Tecnología Agropecuaria (INTA), Hurlingham, Buenos Aires, Argentina
⨯
Victoria Gnazzo,

Affiliations Instituto de Virología, Centro de Investigación en Ciencias Veterinarias y Agronómicas, Instituto Nacional de Tecnología Agropecuaria (INTA), Hurlingham, Buenos Aires, Argentina, Consejo Nacional de Investigaciones Científicas y Tecnológicas, Ciudad Autónoma de Buenos Aires, Buenos Aires, Argentina
⨯
Yves Durocher,

Affiliation Biotechnology Research Institute, National Research Council of Canada, Montreal, Quebec, Canada
⨯
Andrés Wigdorovitz

Affiliations Instituto de Virología, Centro de Investigación en Ciencias Veterinarias y Agronómicas, Instituto Nacional de Tecnología Agropecuaria (INTA), Hurlingham, Buenos Aires, Argentina, Consejo Nacional de Investigaciones Científicas y Tecnológicas, Ciudad Autónoma de Buenos Aires, Buenos Aires, Argentina
⨯

Transient Gene Expression in Serum-Free Suspension-Growing Mammalian Cells for the Production of Foot-and-Mouth Disease Virus Empty Capsids

Ana Clara Mignaqui,
Vanesa Ruiz,
Sylvie Perret,
Gilles St-Laurent,
Parminder Singh Chahal,
Julia Transfiguracion,
Ayelén Sammarruco,
Victoria Gnazzo,
Yves Durocher,
Andrés Wigdorovitz

x

Published: August 20, 2013
https://doi.org/10.1371/journal.pone.0072800

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Transfection
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Capsids
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Recombinant proteins
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Recombinant vaccines
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Viral vaccines
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Structural proteins
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Proteases
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Foot and mouth disease
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