Assessing the Fecal Microbiota: An Optimized Ion Torrent 16S rRNA Gene-Based Analysis Protocol

• Christian Milani ,

  Contributed equally to this work with: Christian Milani, Arancha Hevia

  Affiliation Laboratory of Probiogenomics, Department of Life Sciences, University of Parma, Italy

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• Arancha Hevia ,

  Contributed equally to this work with: Christian Milani, Arancha Hevia

  Affiliation Departamento de Microbiologia y Bioquimica de Productos Lacteos, IPLA – CSIC, Villaviciosa, Asturias, Spain

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• Elena Foroni,

  Affiliation Laboratory of Probiogenomics, Department of Life Sciences, University of Parma, Italy

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• Sabrina Duranti,

  Affiliation Laboratory of Probiogenomics, Department of Life Sciences, University of Parma, Italy

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• Francesca Turroni,

  Affiliation Alimentary Pharmabiotic Centre and Department of Microbiology, Bioscience Institute, National University of Ireland, Western Road, Cork, Ireland

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• Gabriele Andrea Lugli,

  Affiliation Laboratory of Probiogenomics, Department of Life Sciences, University of Parma, Italy

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• Borja Sanchez,

  Affiliation Departamento de Microbiologia y Bioquimica de Productos Lacteos, IPLA – CSIC, Villaviciosa, Asturias, Spain

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• Rebeca Martín,

  Affiliation INRA, UMR 1319 MICALIS-Microbiologie de l’Alimentation au Service de la Santé humaine, Pôle Ecosystèmes: Interactions des bactéries commensales et probiotiques avec l’hôte, Domaine de Vilvert, Bât 440 R-2 78352, Jouy en Josas, France

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• Miguel Gueimonde,

  Affiliation Departamento de Microbiologia y Bioquimica de Productos Lacteos, IPLA – CSIC, Villaviciosa, Asturias, Spain

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• Douwe van Sinderen,

  Affiliation Alimentary Pharmabiotic Centre and Department of Microbiology, Bioscience Institute, National University of Ireland, Western Road, Cork, Ireland

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• Abelardo Margolles ,

  * E-mail: amargolles@ipla.csic.es (AM); marco.ventura@unipr.it (MV)

  Affiliation Departamento de Microbiologia y Bioquimica de Productos Lacteos, IPLA – CSIC, Villaviciosa, Asturias, Spain

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• Marco Ventura

  * E-mail: amargolles@ipla.csic.es (AM); marco.ventura@unipr.it (MV)

  Affiliation Laboratory of Probiogenomics, Department of Life Sciences, University of Parma, Italy

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