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The Impact of Heterogeneity and Dark Acceptor States on FRET: Implications for Using Fluorescent Protein Donors and Acceptors

  • Steven S. Vogel ,

    stevevog@mail.nih.gov

    Affiliation Laboratory of Molecular Physiology, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Bethesda, Maryland, United States of America

  • Tuan A. Nguyen,

    Affiliation Laboratory of Molecular Physiology, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Bethesda, Maryland, United States of America

  • B. Wieb van der Meer,

    Affiliation Department of Physics and Astronomy, Western Kentucky University, Bowling Green, Kentucky, United States of America

  • Paul S. Blank

    Affiliation Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, United States of America

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