About the Authors

Hongshuo Liu

Affiliation Graduate School of Agriculture, Meiji University, Kanagawa, Japan

Wataru Marubashi

marubasi@isc.meiji.ac.jp

Affiliation Graduate School of Agriculture, Meiji University, Kanagawa, Japan

Competing Interests

The authors have the following interests. Although the seeds of N. trigonophylla Dunal (2n = 24), N. tabacum (2n = 48, SSTT) ‘Red Russian,’ N. sylvestris Speg. & Comes (2n = 24, SS) and N. tomentosiformis Goodsp. (2n = 24, TT) used in this experiment were kindly provided free of charge, no types of funding, salaries, payments, honoraria, etc. were received from Japan Tobacco Inc (Oyama, Japan). This does not alter the authors’ adherence to all the PLOS ONE policies on sharing data and materials, as detailed online in the guide for authors.

Author Contributions

Conceived and designed the experiments: WM. Performed the experiments: HL. Analyzed the data: HL WM. Wrote the paper: HL WM.