A Split-Ubiquitin Yeast Two-Hybrid Screen to Examine the Substrate Specificity of atToc159 and atToc132, Two Arabidopsis Chloroplast Preprotein Import Receptors

Siddhartha Dutta; Howard J. Teresinski; Matthew D. Smith

doi:10.1371/journal.pone.0095026

Loading metrics

Open Access

Peer-reviewed

Research Article

A Split-Ubiquitin Yeast Two-Hybrid Screen to Examine the Substrate Specificity of atToc159 and atToc132, Two Arabidopsis Chloroplast Preprotein Import Receptors

Siddhartha Dutta,

Current address: Department of Plant Biology, Michigan State University, East Lansing, Michigan, United States of America

Affiliation Department of Biology, Wilfrid Laurier University, Waterloo, Ontario, Canada
⨯
Howard J. Teresinski,

Current address: Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada

Affiliation Department of Biology, Wilfrid Laurier University, Waterloo, Ontario, Canada
⨯
Matthew D. Smith

* E-mail: msmith@wlu.ca

Affiliation Department of Biology, Wilfrid Laurier University, Waterloo, Ontario, Canada
⨯

A Split-Ubiquitin Yeast Two-Hybrid Screen to Examine the Substrate Specificity of atToc159 and atToc132, Two Arabidopsis Chloroplast Preprotein Import Receptors

Siddhartha Dutta,
Howard J. Teresinski,
Matthew D. Smith

Published: April 15, 2014
https://doi.org/10.1371/journal.pone.0095026

About the Authors

Siddhartha Dutta

Current address: Department of Plant Biology, Michigan State University, East Lansing, Michigan, United States of America

Affiliation Department of Biology, Wilfrid Laurier University, Waterloo, Ontario, Canada

Howard J. Teresinski

Current address: Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada

Affiliation Department of Biology, Wilfrid Laurier University, Waterloo, Ontario, Canada

Matthew D. Smith

* E-mail: msmith@wlu.ca

Affiliation Department of Biology, Wilfrid Laurier University, Waterloo, Ontario, Canada

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: SD MDS. Performed the experiments: SD HJT. Analyzed the data: SD HJT MDS. Contributed reagents/materials/analysis tools: SD MDS. Wrote the paper: SD MDS.

Subject Areas
?

For more information about PLOS Subject Areas, click here.
We want your feedback. Do these Subject Areas make sense for this article? Click the target next to the incorrect Subject Area and let us know. Thanks for your help!

Library screening
Is the Subject Area "Library screening" applicable to this article?

Thanks for your feedback.
Chloroplasts
Is the Subject Area "Chloroplasts" applicable to this article?

Thanks for your feedback.
Yeast
Is the Subject Area "Yeast" applicable to this article?

Thanks for your feedback.
Saccharomyces cerevisiae
Is the Subject Area "Saccharomyces cerevisiae" applicable to this article?

Thanks for your feedback.
Protein interactions
Is the Subject Area "Protein interactions" applicable to this article?

Thanks for your feedback.
DNA libraries
Is the Subject Area "DNA libraries" applicable to this article?

Thanks for your feedback.
Arabidopsis thaliana
Is the Subject Area "Arabidopsis thaliana" applicable to this article?

Thanks for your feedback.
cDNA library screening
Is the Subject Area "cDNA library screening" applicable to this article?

Thanks for your feedback.