About the Authors

Hiroto Sasaki

Affiliation Department of Biotechnology, Graduate School of Engineering, Nagoya University, Nagoya, Aichi, Japan

Ichiro Takeuchi

Affiliation Department of Computer Science/Scientific and Engineering Simulation, Graduate School of Engineering, Nagoya Institute of Technology, Nagoya, Aichi, Japan

Mai Okada

Affiliation Department of Basic Medicinal Sciences, Graduate School of Pharmaceutical Sciences, Nagoya University, Nagoya, Aichi, Japan

Rumi Sawada

Affiliation Division of Medical Devices, National Institute of Health Sciences, Setagaya-ku, Tokyo, Japan

Kei Kanie

Affiliations Department of Biotechnology, Graduate School of Engineering, Nagoya University, Nagoya, Aichi, Japan, Department of Basic Medicinal Sciences, Graduate School of Pharmaceutical Sciences, Nagoya University, Nagoya, Aichi, Japan

Yasujiro Kiyota

Affiliation Nikon Corporation, Chiyoda-ku, Tokyo, Japan

Hiroyuki Honda

Affiliation Department of Biotechnology, Graduate School of Engineering, Nagoya University, Nagoya, Aichi, Japan

Ryuji Kato

kato-r@ps.nagoya-u.ac.jp

Affiliations Department of Biotechnology, Graduate School of Engineering, Nagoya University, Nagoya, Aichi, Japan, Department of Basic Medicinal Sciences, Graduate School of Pharmaceutical Sciences, Nagoya University, Nagoya, Aichi, Japan

Competing Interests

The authors declare there are no competing interests except for the patents and employment stated below. Author RK received a research grant from Nikon Corporation for collaborative research titled “Development of quality assurance technology using cellular images” (18000 US dollars/year). Also, RK is one of the main investigators of 12 patents (pending) related to basic technologies for “algorithms of cellular image processing”. Author YK is the manager of the Business Planning Department, Instrument Company, Nikon Corporation. He is a member of a collaborative project with Nagoya University and is also one of the inventors of 12 related patents. Author HS is one of the main investigators of 2 patents in 12 related patents. Related patents pending: WO 2010/098105, PCT/JP2010/005119, 2010-100162, 2010-100163, 2010-100164, 2010-100165, 2010-100166, 2010-100167, 2011-042210, 2011-096258, 2011-501512, US-2012-0092478-A1. However above all, the authors declare that this does not alter their adherence to PLOS ONE policies on sharing data and materials.

Author Contributions

Conceived and designed the experiments: HS RS KK RK. Performed the experiments: HS MO. Analyzed the data: HS IT RK. Contributed reagents/materials/analysis tools: YK HH RK. Wrote the paper: HS IT RK.