About the Authors
- Yuji Kashiwakura
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Contributed equally to this work with: Yuji Kashiwakura, Jun Mimuro, Akira Onishi, Masaki Iwamoto
Affiliation Research Division of Cell and Molecular Medicine, Center for Molecular Medicine, Jichi Medical University, Shimotsuke, Tochigi-ken, Japan
- Jun Mimuro
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Contributed equally to this work with: Yuji Kashiwakura, Jun Mimuro, Akira Onishi, Masaki Iwamoto
* E-mail: mimuro-j@jichi.ac.jp
Affiliation Research Division of Cell and Molecular Medicine, Center for Molecular Medicine, Jichi Medical University, Shimotsuke, Tochigi-ken, Japan
- Akira Onishi
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Contributed equally to this work with: Yuji Kashiwakura, Jun Mimuro, Akira Onishi, Masaki Iwamoto
Affiliation Transgenic Animal Research Center, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki-ken, Japan
- Masaki Iwamoto
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Contributed equally to this work with: Yuji Kashiwakura, Jun Mimuro, Akira Onishi, Masaki Iwamoto
Affiliations Transgenic Animal Research Center, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki-ken, Japan, Advanced Technology Development Team, Prime Tech Ltd., Tsuchiura, Ibaraki-ken, Japan
- Seiji Madoiwa
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Affiliation Research Division of Cell and Molecular Medicine, Center for Molecular Medicine, Jichi Medical University, Shimotsuke, Tochigi-ken, Japan
- Daiichiro Fuchimoto
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Affiliation Transgenic Animal Research Center, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki-ken, Japan
- Shunichi Suzuki
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Affiliation Transgenic Animal Research Center, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki-ken, Japan
- Misae Suzuki
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Affiliation Transgenic Animal Research Center, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki-ken, Japan
- Shoichiro Sembon
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Affiliation Transgenic Animal Research Center, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki-ken, Japan
- Akira Ishiwata
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Affiliation Research Division of Cell and Molecular Medicine, Center for Molecular Medicine, Jichi Medical University, Shimotsuke, Tochigi-ken, Japan
- Atsushi Yasumoto
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Affiliation Research Division of Cell and Molecular Medicine, Center for Molecular Medicine, Jichi Medical University, Shimotsuke, Tochigi-ken, Japan
- Asuka Sakata
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Affiliation Research Division of Cell and Molecular Medicine, Center for Molecular Medicine, Jichi Medical University, Shimotsuke, Tochigi-ken, Japan
- Tsukasa Ohmori
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Affiliation Research Division of Cell and Molecular Medicine, Center for Molecular Medicine, Jichi Medical University, Shimotsuke, Tochigi-ken, Japan
- Michiko Hashimoto
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Affiliation Advanced Technology Development Team, Prime Tech Ltd., Tsuchiura, Ibaraki-ken, Japan
- Satoko Yazaki
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Affiliation Advanced Technology Development Team, Prime Tech Ltd., Tsuchiura, Ibaraki-ken, Japan
- Yoichi Sakata
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Affiliation Research Division of Cell and Molecular Medicine, Center for Molecular Medicine, Jichi Medical University, Shimotsuke, Tochigi-ken, Japan
Competing Interests
MI, MH, and SY are employees of Prim Tech Ltd., the developer of Piezo micromanipulators for nuclear transfer, and are collaborative with National Institute of Agrobiological Sciences and Jichi Meidical University for basic research. Other authors declare no conflict of interest. This does not alter the authors adherence to all the PLOS ONE policies on sharing data and materials.
Author Contributions
Wrote the paper: JM. Senior investigator and supervised this study: YS. Acted as a senior investigator, planned, generated constructs for F8 gene targeting, and conducted the study: JM. Postdoctoral fellow, conducted most of the F8 targeting work and injected human FVIII into a cloned piglet: YK. Performed nuclear transfer of targeted cells to oocytes and transplantation of the oocytes, and managed care of cloned pigs: AO MI. Injected human FVIII into a cloned piglet on day 1 after birth: SM. Cared for cloned piglets and did pathological examination of cloned piglets: DF SS MS SS MH SY. Performed cell culture experiments: AI AY AS TO.