About the Authors

Tien-chen Lin

Affiliation Zentrum für Molekulare Biologie der Universität Heidelberg, DKFZ-ZMBH Allianz, Heidelberg, Germany

Linda Gombos

Affiliation Zentrum für Molekulare Biologie der Universität Heidelberg, DKFZ-ZMBH Allianz, Heidelberg, Germany

Annett Neuner

Affiliation Zentrum für Molekulare Biologie der Universität Heidelberg, DKFZ-ZMBH Allianz, Heidelberg, Germany

Dominik Sebastian

Current address: DKFZ, Heidelberg, Germany

Affiliation Zentrum für Molekulare Biologie der Universität Heidelberg, DKFZ-ZMBH Allianz, Heidelberg, Germany

Jesper V. Olsen

Current address: Novo Nordisk Foundation Center for Protein Research, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark

Affiliation MPI Biochemistry, Martinsried, Germany

Ajla Hrle

Affiliation MPI Biochemistry, Martinsried, Germany

Christian Benda

Affiliation MPI Biochemistry, Martinsried, Germany

Elmar Schiebel

schiebel.elmar@zmbh.uni-heidelberg.de

Affiliation Zentrum für Molekulare Biologie der Universität Heidelberg, DKFZ-ZMBH Allianz, Heidelberg, Germany

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: T-cL. Performed the experiments: T-cL. Analyzed the data: T-cL. Contributed reagents/materials/analysis tools: T-cL. Wrote the paper: ES. Thin section electron microscopy: AN. Construction of TUB4, SPC97 and SPC98 mutants: DS T-cL. Construction of Tub4-AID degron system: LG. Spc97-TAP purification and MS/MS analysis: CB JVO AH.