A Comparison of Donor-Acceptor Pairs for Genetically Encoded FRET Sensors: Application to the Epac cAMP Sensor as an Example

Gerard N. M. van der Krogt; Janneke Ogink; Bas Ponsioen; Kees Jalink

doi:10.1371/journal.pone.0001916

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Open Access

Peer-reviewed

Research Article

A Comparison of Donor-Acceptor Pairs for Genetically Encoded FRET Sensors: Application to the Epac cAMP Sensor as an Example

Gerard N. M. van der Krogt,

Affiliation Division of Cell Biology, The Netherlands Cancer Institute, Amsterdam, The Netherlands
⨯
Janneke Ogink,

Affiliation Division of Cell Biology, The Netherlands Cancer Institute, Amsterdam, The Netherlands
⨯
Bas Ponsioen,

Affiliations Division of Cell Biology, The Netherlands Cancer Institute, Amsterdam, The Netherlands, Division of Cellular Biochemistry, The Netherlands Cancer Institute, Amsterdam, The Netherlands
⨯
Kees Jalink

*To whom correspondence should be addressed. E-mail: k.jalink@nki.nl

Affiliation Division of Cell Biology, The Netherlands Cancer Institute, Amsterdam, The Netherlands
⨯

A Comparison of Donor-Acceptor Pairs for Genetically Encoded FRET Sensors: Application to the Epac cAMP Sensor as an Example

Gerard N. M. van der Krogt,
Janneke Ogink,
Bas Ponsioen,
Kees Jalink

Published: April 2, 2008
https://doi.org/10.1371/journal.pone.0001916

About the Authors

Gerard N. M. van der Krogt

Affiliation Division of Cell Biology, The Netherlands Cancer Institute, Amsterdam, The Netherlands

Janneke Ogink

Affiliation Division of Cell Biology, The Netherlands Cancer Institute, Amsterdam, The Netherlands

Bas Ponsioen

Affiliations Division of Cell Biology, The Netherlands Cancer Institute, Amsterdam, The Netherlands, Division of Cellular Biochemistry, The Netherlands Cancer Institute, Amsterdam, The Netherlands

Kees Jalink

*To whom correspondence should be addressed. E-mail: k.jalink@nki.nl

Affiliation Division of Cell Biology, The Netherlands Cancer Institute, Amsterdam, The Netherlands

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: KJ Gv. Performed the experiments: KJ Gv JO BP. Analyzed the data: KJ Gv BP. Contributed reagents/materials/analysis tools: KJ Gv. Wrote the paper: KJ Gv.

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Fluorescence resonance energy transfer
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Yellow fluorescent protein
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Fluorescence
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Fluorescence imaging
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Bandpass filters
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Dimerization
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Reporter gene assay
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Imaging techniques
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