A Fully Automated Robotic System for Microinjection of Zebrafish Embryos

Wenhui Wang; Xinyu Liu; Danielle Gelinas; Brian Ciruna; Yu Sun

doi:10.1371/journal.pone.0000862

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Open Access

Peer-reviewed

Research Article

A Fully Automated Robotic System for Microinjection of Zebrafish Embryos

Wenhui Wang ,

Contributed equally to this work with: Wenhui Wang, Xinyu Liu

Affiliation Advanced Micro and Nanosystems Laboratory, University of Toronto, Toronto, Canada
⨯
Xinyu Liu ,

Contributed equally to this work with: Wenhui Wang, Xinyu Liu

Affiliation Advanced Micro and Nanosystems Laboratory, University of Toronto, Toronto, Canada
⨯
Danielle Gelinas,

Affiliation Program in Developmental and Stem Cell Biology, The Hospital for Sick Children, Toronto, Canada
⨯
Brian Ciruna ,

*To whom correspondence should be addressed. E-mail: ciruna@sickkids.ca (BC); sun@mie.utoronto.ca (YS)

Affiliation Program in Developmental and Stem Cell Biology, The Hospital for Sick Children, Toronto, Canada
⨯
Yu Sun

*To whom correspondence should be addressed. E-mail: ciruna@sickkids.ca (BC); sun@mie.utoronto.ca (YS)

Affiliation Advanced Micro and Nanosystems Laboratory, University of Toronto, Toronto, Canada
⨯

A Fully Automated Robotic System for Microinjection of Zebrafish Embryos

Wenhui Wang,
Xinyu Liu,
Danielle Gelinas,
Brian Ciruna,
Yu Sun

Published: September 12, 2007
https://doi.org/10.1371/journal.pone.0000862

About the Authors

Wenhui Wang

Contributed equally to this work with: Wenhui Wang, Xinyu Liu

Affiliation Advanced Micro and Nanosystems Laboratory, University of Toronto, Toronto, Canada

Xinyu Liu

Contributed equally to this work with: Wenhui Wang, Xinyu Liu

Affiliation Advanced Micro and Nanosystems Laboratory, University of Toronto, Toronto, Canada

Danielle Gelinas

Affiliation Program in Developmental and Stem Cell Biology, The Hospital for Sick Children, Toronto, Canada

Brian Ciruna

*To whom correspondence should be addressed. E-mail: ciruna@sickkids.ca (BC); sun@mie.utoronto.ca (YS)

Affiliation Program in Developmental and Stem Cell Biology, The Hospital for Sick Children, Toronto, Canada

Yu Sun

*To whom correspondence should be addressed. E-mail: ciruna@sickkids.ca (BC); sun@mie.utoronto.ca (YS)

Affiliation Advanced Micro and Nanosystems Laboratory, University of Toronto, Toronto, Canada

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: YS. Performed the experiments: WW XL. Analyzed the data: WW XL. Contributed reagents/materials/analysis tools: DG BC. Wrote the paper: YS.

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Embryos
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Zebrafish
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Cytoplasm
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Fluorescent dyes
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Chorion
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Material fatigue
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Embryonic pattern formation
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Technicians
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