Reader Comments

Post a new comment on this article

General annotation

Posted by giacomoc on 09 Sep 2007 at 07:58 GMT

can be imaged in 3D using OPT, also we show that this method of clearing is also suitable for confocal microscopy to image the brain from within the intact head. The major advantage of this is that organs can be represented in 3D in their natural surroundings. Furthermore optical sections are generated in each of the three planes and are not prone to the technical limitations that are associated with manual sectioning. OPT can be used to dissect mutant phenotypes and to globally map gene expression in both 2D and 3D
http://plosone.org/article/info:doi/10.1371/journal.pone.0000834#article1.front1.article-meta1.abstract1.sec3.p1

This is an exciting paper, very useful to many people in the field.

What can the author say about the maximal spatial resolution one can get? Are subcellular 3D structures preserved by the treatment?

report a concern respond to this posting