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closeReferee Comments: Referee 2 (Linheng Li)
Posted by PLOS_ONE_Group on 05 Oct 2007 at 21:15 GMT
Reviewer 2's Review (Linheng Li)
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This paper investigates the effects of canonical Wnt signaling on gene expression within human lung fibroblasts. The authors compare gene expression, using DNA microarray, in human lung fibroblasts that have been incubated with and without Wnt3a. Using this data, they hypothesize about the roles of Wnt induced signaling within tissue specific fibroblasts that may contribute to micro environmental regulation of epithelial stem cells. The microarray analysis indicated that an increase in Gremlin, a BMP antagonist, is significantly upregulated after Wnt induction providing a potential mechanism for Wnt mediated mesenchymal involvement in stem cell regulation. While the data presented provides interesting and useful information, more work may be needed prior to presenting the signaling models and suppositions described in this paper.
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N.B. These are the general comments made by the reviewer when reviewing this paper in light of which the manuscript was revised. Specific points addressed during revision of the paper are not shown.