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Research Article

Whole-Genome Sequencing and Assembly with High-Throughput, Short-Read Technologies

  • Andreas Sundquist mail,

    To whom correspondence should be addressed. E-mail: asundqui@cs.stanford.edu

    Affiliation: Department of Computer Science, Stanford University, Stanford, California, United States of America

    X
  • Mostafa Ronaghi,

    Affiliation: Stanford Genome Technology Center, Stanford, California, United States of America

    X
  • Haixu Tang,

    Affiliation: School of Informatics, Indiana University, Bloomington, Indiana, United States of America

    X
  • Pavel Pevzner,

    Affiliation: Department of Computer Science and Engineering, University of California, San Diego, La Jolla, California, United States of America

    X
  • Serafim Batzoglou

    Affiliation: Department of Computer Science, Stanford University, Stanford, California, United States of America

    X
  • Published: May 30, 2007
  • DOI: 10.1371/journal.pone.0000484

Reader Comments (6)

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How feasible is this?

Posted by hctu on 08 Jun 2007 at 08:07 GMT

10±2 Kb and 40±8 Kb
http://plosone.org/article/info:doi/10.1371/journal.pone.0000484#article1.body1.sec3.sec4.p2

At JGI we have a hard time generating paired Solexa reads with this kind of insert sizes. Current strategy relies on the ditag approach, which creates ~3kb insert sizes.