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closePG-M3 is not PML specific
Posted by TheBishopofTurkey on 04 Jun 2008 at 16:21 GMT
Localization of PML was examined by indirect immunofluorescence microscopy in mock infected, infected (1.0 or 0.1 PFU/cell HSV-1) and IFN-γ treated MRC5 cells. Cells were fixed 24h post treatment and immunostained with anti-PML (PG-M3) and Texas- Red conjugated secondary antibody. DAPI was used to counter-stain the nucleus
http://plosone.org/article/info:doi/10.1371/journal.pone.0002277#pone-0002277-g001
You cannot use the antibody PG-M3 to stain for PML during infection by HSV-1 as PG-M3 cross-reacts with ICP4 (see Boulware SL and Weber PC (2000) Cross-reactivity of the anti-PML antibody PG-M3 with the herpes simplex virus type 1 immediate early protein ICP4. J Gen Virol 81: 1773-1777 http://vir.sgmjournals.org/cgi/content/full/81/7/1773)
RE: PG-M3 is not PML specific
zhengp01 replied to TheBishopofTurkey on 09 Jun 2008 at 14:23 GMT
Yes, we are fully aware about the cross reaction of PG-M3. In some of the experiments, we used V5 or c-myc tagged PML Ib and used anti-V5 in IF and confirmed the results.