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Research Article

Observation of Live Ticks (Haemaphysalis flava) by Scanning Electron Microscopy under High Vacuum Pressure

  • Yasuhito Ishigaki,

    Affiliation: Medical Research Institute, Kanazawa Medical University, Uchinada-machi, Kahoku-gun, Japan

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  • Yuka Nakamura,

    Affiliation: Medical Research Institute, Kanazawa Medical University, Uchinada-machi, Kahoku-gun, Japan

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  • Yosaburo Oikawa,

    Affiliation: Department of Medical Zoology, Kanazawa Medical University, Uchinada-machi, Kahoku-gun, Japan

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  • Yasuhiro Yano,

    Affiliation: Department of Pathological Sciences, Faculty of Medical Sciences, University of Fukui, Matsuoka Fukui, Japan

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  • Susumu Kuwabata,

    Affiliations: Department of Applied Chemistry, Graduate School of Engineering, Osaka University, Suita, Osaka, Japan, Japan Science and Technology Agency (JST), Core Research for Evolutional Science and Technology (CREST), Kawaguchi, Saitama, Japan

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  • Hideaki Nakagawa,

    Affiliation: Medical Research Institute, Kanazawa Medical University, Uchinada-machi, Kahoku-gun, Japan

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  • Naohisa Tomosugi mail,

    tomosugi@kanazawa-med.ac.jp

    Affiliation: Medical Research Institute, Kanazawa Medical University, Uchinada-machi, Kahoku-gun, Japan

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  • Tsutomu Takegami

    Affiliation: Medical Research Institute, Kanazawa Medical University, Uchinada-machi, Kahoku-gun, Japan

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  • Published: March 14, 2012
  • DOI: 10.1371/journal.pone.0032676

Reader Comments (2)

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not the first time

Posted by gspaul on 27 Mar 2012 at 19:35 GMT

To our knowledge, this is the first example demonstrating observation of living organisms using SEM in this field.
http://plosone.org/article/info:doi/10.1371/journal.pone.0032676#article1.body1.sec1.p3

This is a nice paper but the premise that this is the first time that living specimens have been viewed with a SEM is incorrect, in 1977, David Scharf wrote a book called Magnifications in which he explained his technique for capturing images of living specimens including many excellent photos. (Schocken Books).

No competing interests declared.

RE: not the second time, either

Ceropegius replied to gspaul on 04 Apr 2012 at 02:40 GMT

I checked PubMed and found these from 1967:

Electron microscopy of living insects.
R F Pease, T L Hayes, A S Camp, N M Amer
Electron micrographs of living specimens of the various developmental stages of the insect Tribolium confusum have been obtained with a scanning electron microscope. In most cases the specimens resumed their normal activity after being examined with the electron microscope and under went metamorphosis into the next stage.
Science. 1967 Jul 28;157 (787):443-5 6028030

Stereoscopic scanning electron microscopy of living Tribolium confusum
by Thomas L Hayes, R F W Pease, Ann S Camp
The scanning electron microscope has been used to produce stereoscopic pictures of living Tribolium confusum at magnifications up to 1300 times. The physiological implications of survival in the environment of electron beam scanning are discussed.
Journal of Insect Physiology (1967)
Volume: 13, Issue: 8, Pages: 1143-1144, IN1-IN2, 1145

No competing interests declared.

RE: RE: not the second time, either

bgoldstein replied to Ceropegius on 04 Apr 2012 at 14:01 GMT

Insects, mites and tardigrades have survived EM, but have any of them been seen to move while under the beam?

No competing interests declared.