Next Generation MUT-MAP, a High-Sensitivity High-Throughput Microfluidics Chip-Based Mutation Analysis Panel

Erica B. Schleifman; Rachel Tam; Rajesh Patel; Alison Tsan; Teiko Sumiyoshi; Ling Fu; Rupal Desai; Nancy Schoenbrunner; Thomas W. Myers; Keith Bauer; Edward Smith; Rajiv Raja

doi:10.1371/journal.pone.0090761

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Open Access

Peer-reviewed

Research Article

Next Generation MUT-MAP, a High-Sensitivity High-Throughput Microfluidics Chip-Based Mutation Analysis Panel

Erica B. Schleifman ,

Contributed equally to this work with: Erica B. Schleifman, Rachel Tam

Affiliation Oncology Biomarker Development, Genentech Inc., South San Francisco, California, United States of America
⨯
Rachel Tam ,

Contributed equally to this work with: Erica B. Schleifman, Rachel Tam

Affiliation Oncology Biomarker Development, Genentech Inc., South San Francisco, California, United States of America
⨯
Rajesh Patel,

Affiliation Oncology Biomarker Development, Genentech Inc., South San Francisco, California, United States of America
⨯
Alison Tsan,

Affiliation Chemistry and Innovation Technology, Pleasanton, California, United States of America
⨯
Teiko Sumiyoshi,

Affiliation Oncology Biomarker Development, Genentech Inc., South San Francisco, California, United States of America
⨯
Ling Fu,

Affiliation Oncology Biomarker Development, Genentech Inc., South San Francisco, California, United States of America
⨯
Rupal Desai,

Affiliation Oncology Biomarker Development, Genentech Inc., South San Francisco, California, United States of America
⨯
Nancy Schoenbrunner,

Affiliation Chemistry and Innovation Technology, Pleasanton, California, United States of America
⨯
Thomas W. Myers,

Affiliation Program in Core Research, Roche Molecular Systems Inc., Pleasanton, California, United States of America
⨯
Keith Bauer,

Affiliation Program in Core Research, Roche Molecular Systems Inc., Pleasanton, California, United States of America
⨯
Edward Smith,

Affiliation Program in Core Research, Roche Molecular Systems Inc., Pleasanton, California, United States of America
⨯
Rajiv Raja

* E-mail: raja.rajiv@gene.com

Affiliation Oncology Biomarker Development, Genentech Inc., South San Francisco, California, United States of America
⨯

Next Generation MUT-MAP, a High-Sensitivity High-Throughput Microfluidics Chip-Based Mutation Analysis Panel

Erica B. Schleifman,
Rachel Tam,
Rajesh Patel,
Alison Tsan,
Teiko Sumiyoshi,
Ling Fu,
Rupal Desai,
Nancy Schoenbrunner,
Thomas W. Myers,
Keith Bauer

Published: March 21, 2014
https://doi.org/10.1371/journal.pone.0090761

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Correction 13 May 2014

Posted by schleif2 on 13 May 2014 at 17:43 GMT

ACKNOWLEDGEMENTS
We would like to thank Suresh Selvaraj for providing genomic DNA from all of the cell lines analyzed in the manuscript. We would also like to thank Anna Faarborg, Mark Lackner, Timothy Wilson, Jill Spoerke, Ashi Malekafzali, Omar Kabbarah, and Doris Kim for making valuable clinical samples available to us to that made this work possible. Further, we would like to thank Chery Victoria Wong, Ilma Abbas, Rin Nakamura, Arjan Gower, Anna Cheung, and My Vo for their efforts in processing clinical samples, DNA isolations, and sample management.

No competing interests declared.

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Next Generation MUT-MAP, a High-Sensitivity High-Throughput Microfluidics Chip-Based Mutation Analysis Panel

Next Generation MUT-MAP, a High-Sensitivity High-Throughput Microfluidics Chip-Based Mutation Analysis Panel

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Correction 13 May 2014

Posted by schleif2 on 13 May 2014 at 17:43 GMT